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- W971973281 abstract "目的观察Ser202/Thr205位点的过度磷酸化tau在神经元中的定位和聚集情况。方法取孕18 d SD胎鼠皮层神经元进行原代培养,于培养第9天加入冈田酸（okadaic acid,OA）处理12 h以诱导tau蛋白磷酸化;同时用OA诱导SH-SY5Y细胞;观察tau磷酸化后神经元形态的变化;用AT-8抗体免疫荧光染色观察Ser202/Thr205位点磷酸化tau的定位和聚集;同时应用AT-8抗体和Tau-5抗体检测tau蛋白Ser202/Thr205位点的磷酸化水平变化情况。结果经过Anti-NeuN和Anti-GFAP免疫染色鉴定,孕18 d胎鼠皮层神经元选择性原代培养成功。OA处理12 h以后,Ser202/Thr205位点磷酸化tau的水平明显增加,且出现高分子量的寡聚体;免疫荧光染色发现,Ser202/Thr205位点磷酸化的tau在原代培养神经元的胞质聚集;而OA处理的SH-SY5Y细胞,Ser202/Thr205位点磷酸化的tau却定位于细胞核。同时发现OA处理的原代神经元突起明显减少或消失。结论 OA能够诱导原代培养的胎鼠神经元tau蛋白在Ser202/Thr205位点过度磷酸化,且该磷酸化tau在不同类型或来源的神经元中的定位不同。" @default.
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- W971973281 date "2011-01-01" @default.
- W971973281 modified "2023-09-23" @default.
- W971973281 title "Ser^202/Thr^205位点磷酸化tau在神经元中的定位" @default.
- W971973281 hasPublicationYear "2011" @default.
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