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- W973164585 abstract "MYB, which plays an important role in many aspects, is one of the biggest families of transcription factor. Niedzwetzkyana schneid, a wild red flesh apple, was employed as the experimental material to clone MYB transcription factors. One of the MYB transcription factors, named MpMYB30 was cloned by RACE technology from the leaves of niedzwetzkyana schneid. The result showed that the total length of MpMYB30 gene was 1661 bp. Bioinformatics analysis showed that the protein of MpMYB30 gene encoded had two MYB HTH DNA-binding domains, which shared compatibility with Vitis viniferna and Ricinus communis: the protein's molecular formula was C1790H2794N534O584S12 and the relative molecular weight was 41579.8 ku; the isoelectric point was 6.30, and contained more α-helix in its secondary structure, but did not existed a signal peptide and transmembrane regions an its multi-peptide. The sequence of MpMYB30 gene was digested by Xbal Ⅰ and Sac Ⅰ, then linked into pCAMBIA-S1300+vector, after that it was confirmed by resistance selected and PCR analysis." @default.
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- W973164585 date "2010-07-10" @default.
- W973164585 modified "2023-09-23" @default.
- W973164585 title "红肉苹果(Malus pumila var. niedzwetzkyana)MpMYB30基因的克隆及表达载体的构建" @default.
- W973164585 hasPublicationYear "2010" @default.
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