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- W973744933 abstract "This chapter describes the use of the λ phage promoter PL to promote gene expression in hybrid-plasmid-cloning vehicles. The expression of prokaryotic structural genes in bacteria is dependent on the deoxyribonucleic acid (DNA) sequences upstream from these genes that do not code for a polypeptide chain, for example, promoters, operators, and ribosome-binding sites. Physical separation in vitro of a particular structural gene and its regulatory sequences may be possible by the cleavage of the DNA with a suitable restriction endonuclease at a restriction site between these two DNA sequences. This chapter describes the construction and use in Escherichia coli (E. coli) of plasmid cloning vehicles designed to promote the expression of gene inserts. DNA fragments that contain genes deleted for their own promoters can be inserted into any one of the four different single restriction sites on these vehicles. The transcription of these genes occurs from the strong phage λ promoter PL located on the vehicles, from which ribonucleic acid (RNA) polymerase can read into the inserted fragments. These vehicles should be useful in promoting high levels of expression of prokaryotic genes that are weakly expressed in their natural genetic environment or in promoting the transcription of eukaryotic genes that are poorly expressed in E. coli." @default.
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- W973744933 date "1979-01-01" @default.
- W973744933 modified "2023-10-03" @default.
- W973744933 title "[35] Use of the λ phage promoter PL to promote gene expression in hybrid plasmid cloning vehicles" @default.
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- W973744933 doi "https://doi.org/10.1016/0076-6879(79)68037-0" @default.
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