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- W974101047 abstract "The approaches used for positionally cloning a disease gene from a defined chromosomal region are rapidly evolving. In the past this step began with the screening of yeast artificial chromosome (YAC) libraries, followed by the identification of smaller clones propagated in Escherichia coli. With increasing amounts of resources generated by large-scale approaches, and advertised over the World Wide Web, this step in a positional cloning procedure may no longer be necessary. Transcripts can be identified using a variety of different methods, including those performed at the bench and those performed “in silico.” Each of the genes identified need to be assessed for involvement in the disease. After the correct disease gene has been cloned, further functional analyses are often necessary to understand the role of the encoded protein and the pathophysiological mechanisms involved in the disease process." @default.
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- W974101047 date "2000-01-01" @default.
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- W974101047 title "The Identification of Disease Genes in a Candidate Region" @default.
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- W974101047 doi "https://doi.org/10.1007/978-1-59259-033-9_21" @default.
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