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- W974214253 abstract "Publisher Summary This chapter discusses the assays, occurrence, and specificity of glutamate carboxylase. The formation of γ-carboxyglutamate in proteins is catalyzed by a microsomal enzyme system that requires vitamin K as a cofactor. The chapter discusses the preparation of microsomal enzyme. Microsomes are prepared from the postmitochondrial supernatant by centrifugation at 105,000 g for 60 min, and the microsomal pellet is resuspended with 8 strokes of a loose-fitting Dounce homogenizer, followed by the repelleting of the microsomes. The washed microsomes are solubilized at 4 ° by suspension with the glass homogenizer in buffer A containing 1.0% Triton X-100. The suspension is left at 4° for 30 min and the solubilized protein is clarified by ultracentrifugation. After solubilization, the concentration of detergent may be lowered by dilution or attempts to purify carboxylase, and a detergent requirement for the expression of maximal enzyme activity may become apparent. The available data suggests that macromolecular recognition sites are more important than any primary sequence consideration in determining the specificity of glutamate carboxylase for particular Glu residues in a protein." @default.
- W974214253 created "2016-06-24" @default.
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- W974214253 date "1984-01-01" @default.
- W974214253 modified "2023-09-27" @default.
- W974214253 title "Glutamate carboxylase: Assays, occurrence, and specificity" @default.
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- W974214253 doi "https://doi.org/10.1016/0076-6879(84)07039-7" @default.
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