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- W97448508 abstract "Publisher Summary This chapter describes the preparation of a cell-free protein-synthesizing system from wheat germ. Crude extracts of commercial wheat germ are known to be capable of translating a wide variety of messenger RNAs. Most wheat germ extracts have low endogenous activity. If necessary, endogenous activity may be further reduced by a preincubation step with creatine phosphokinase or by treatment with micrococcal nuclease. Even with extracts that have a relatively high endogenous activity as determined by the incorporation of radioactive amino acids, few if any endogenous protein bands are observed on SDS-polyacrylamide gels. HEPES should be stored frozen in small aliquots; it supports microbial growth and it is not stable to autoclaving. One should also remember that the buffer supplies some potassium that has not been accounted for in the concentrations given." @default.
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- W97448508 date "1983-01-01" @default.
- W97448508 modified "2023-10-14" @default.
- W97448508 title "[41] Preparation of a cell-free protein-synthesizing system from wheat germ" @default.
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- W97448508 doi "https://doi.org/10.1016/0076-6879(83)01044-7" @default.
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