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- W975436475 abstract "Publisher Summary This chapter discusses the isolation and assay of a phosphodiesterase inhibitor from retinal rod outer segments. Photolyzed rhodopsin activates a cyclic guanosine monophosphate (cGMP) phosphodiesterase in rod outer segments (ROS) in the presence of guanosine triphosphate (GTP). It has been proposed that photolyzed rhodopsin, GTP, and light-activated GTPase counteract the effects of this inhibitor on the phosphodiesterase. The most effective method for isolating this inhibitor takes advantage of its tight binding to the phosphodiesterase. The inhibitor copurifies with the phosphodiesterase and can then be isolated from the phosphodiesterase by heat treatment. Heat treatment was used to isolate the inhibitor from the phosphodiesterase. The highest yields of inhibitor were obtained by treating 0.6 mg/ml purified phosphodiesterase at pH 12.0 for 5 min at 100°. The pH was then lowered to 5.9 to precipitate the denatured phosphodiesterase." @default.
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- W975436475 date "1982-01-01" @default.
- W975436475 modified "2023-09-26" @default.
- W975436475 title "[73] Isolation and assay of a phosphodiesterase inhibitor from retinal rod outer segments" @default.
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- W975436475 doi "https://doi.org/10.1016/s0076-6879(82)81075-6" @default.
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