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- W976984911 abstract "This chapter discusses the procedure for the isolation of leaf peroxisomes. Leaf peroxisomes are respiratory microbodies present in the leaves of all plants. In appearance, size, and enzymatic composition, leaf peroxisomes belong to a class of microbodies that are similar to peroxisomes from liver and kidney, and they are similar but not identical with glyoxysomes from germinating seed endosperm. The main steps in the procedure for the isolation of leaf peroxisomes are (1) grinding of the leaves; (2) differential centrifugation between 100 and 6800 g for partial separation and for enrichment of the particulate fraction; (3) an isopycnic, nonlinear sucrose density gradient centrifugation at 39,000 g; and (4) a linear sucrose density gradient centrifugation. The first nonlinear sucrose density gradient acts as a filtration process in which the chloroplasts and mitochondria are stopped by layers of sucrose between 1.3 and 1.75 M . The peroxisomes are trapped at an interface below 1.75 M sucrose generally on top of 2.3 M sucrose. In isolating leaf peroxisomes, a major difference encountered from work with liver or seed endosperm tissue is the immense bulk of chloroplasts from which the peroxisomes must be separated." @default.
- W976984911 created "2016-06-24" @default.
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- W976984911 date "1971-01-01" @default.
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- W976984911 title "[65] Isolation of leaf peroxisomes" @default.
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- W976984911 doi "https://doi.org/10.1016/s0076-6879(71)23141-4" @default.
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