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- W977137792 abstract "目的 探讨核苷酸切除修复基因ERCC1在肺癌细胞修复苯并 (a)芘所致DNA损伤中的作用。方法 构建表达ERCC1反义RNA的重组质粒 ,Lipofectin转染肺癌A5 4 9细胞 ,潮霉素筛选出稳定转染的细胞克隆 ;噻唑蓝法检测 2 4h细胞生存力 ;NorthernBlot分析细胞ERCC1基因mRNA表达水平 ;单细胞凝胶电泳技术检测DNA损伤程度 ,每组计算 5 0个细胞损伤情况。结果 筛选出表达ERCC1反义RNA的 7个阳性克隆 ,其生长特性与亲本细胞差别无显著性 ;内源性mRNA表达水平不同程度降低 ,为亲本细胞的 12 %～ 86 % ;DNA损伤修复速度减慢 ,10 μmol/L苯并 (a)芘作用 2 4h后再孵育 2 4h ,修复程度为亲本细胞的 2 9%～ 71% ;相关分析表明DNA损伤修复程度与ERCC1mRNA水平显著相关。结论 ERCC1反义RNA降低肺癌细胞对苯并 (a)芘所致DNA损伤的修复能力。" @default.
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- W977137792 date "2003-05-06" @default.
- W977137792 modified "2023-09-25" @default.
- W977137792 title "ERCC1反义RNA降低肺癌细胞对苯并（a）芘所致DNA损伤的修复能力" @default.
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