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- W977320925 abstract "This chapter focuses on study of protein-protein and of protein-ligand interactions by potentiometric methods. This application of potentiometric technique is described in this chapter. Protein–protein or protein–ligand association reactions are frequently pH dependent. It is equivalent to state that hydrogen ions are released or bound as these reactions take place or that the titration curves of the products differ from the titration curves of the reactants. The chapter discusses the methods of direct measurement of the difference titration curves for reactions of the form A + B → C, where A is a protein and B may be either a protein or a ligand that interacts with A to form a 1:1 complex. The chapter describes the method of measurement of large Kassoc that bears a close relationship to the methods of measurement of very small Km's, where inconveniently small substrate concentrations would have to be employed in direct measurement and therefore competitive inhibitors are added." @default.
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- W977320925 date "1972-01-01" @default.
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- W977320925 title "[10] Study of protein-protein and of protein-ligand interactions by potentiometric methods" @default.
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- W977320925 doi "https://doi.org/10.1016/s0076-6879(72)26012-8" @default.
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