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- W977492747 abstract "Publisher Summary This chapter describes procedure for production of interferon by induction of KG-1 cells with Sendai virus. This procedure has been useful in obtaining human leukocyte interferon for characterization and interferon mRNA used for cloning. To maintain the KG-1 cells in log phase (0.5 to 1.0×106 cells/ml), the cultures are passaged twice weekly at a splitting ratio of 1:2. A peristaltic pump equipped with silicon tubing and modified glass filling bells is used to split the cultures, add fresh culture medium, and harvest the KG-1 cells for interferon induction. Sixty consecutive inductions of KG-I cells have produced l×10 TM units of interferon. Each induction consisted of about 12 liters of concentrated KG-I cells and yielded an average interferon titer of 2 x l0 5 reference units of interferon per 10 7 cells. An 80-fold concentration of the induction medium with an 80-100% recovery of the interferon activity is obtained by the concentration procedure." @default.
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- W977492747 date "1981-01-01" @default.
- W977492747 modified "2023-09-27" @default.
- W977492747 title "[11] Induction, production, and concentration of interferon produced by a myeloblast culture" @default.
- W977492747 cites W2032819570 @default.
- W977492747 doi "https://doi.org/10.1016/0076-6879(81)78101-1" @default.
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