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- W977857038 abstract "[Objective] To establish an indirect ELISA method which can detect fowl adenovirus group I (FAVI) antibody easily and rapidly. [Method] The expressed and purified FAVI penton recombinant protein was used to be an antigen, optimized the reaction conditions, and then established the FAVI indirect penton-ELISA antibody detection method. [Result] The optimal coating concentration of antigen was 1.5 μg/hole, the optimal coating condition was 37 ℃ 2 h and 4 ℃ overnight; the optimal dilution of serum was 1∶ 100; the optimal working concentration of anti-chicken IgG-HRP was 1∶2 000; the positive and negative critical value of ELISA was 0.335. Detected the 100 chicken serum samples by the established penton-ELISA method, the positive rate was 41%. [Conclusion] Through the study, the established penton-ELISA method has a good specificity, sensitivity and reproducibility. And it offers an effective tool for the diagnosis of FAVI, the survey of antibody and epidemiology survey." @default.
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- W977857038 date "2012-01-01" @default.
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- W977857038 title "Establishment of indirect ELISA method for detection of penton protein of fowl adenovirus group I." @default.
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