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- W977996467 abstract "Publisher Summary This chapter focuses on Urocanase (Pseudomonas putida). The chapter begins with a discussion on Assay method. A unit of urocanase activity is defined as the amount of enzyme catalyzing the disappearance of 1 micromole of urocanate per minute under the above conditions. This unit is based on a molar extinction coefficient of 18,800 for urocanate at pH 7.4 and 277 mμ. A more exact procedure, described by Swaine, corrects for the absorption because of imidazolone propionate formed during the reaction. For routine assays of brief duration, this correction is not required. Specific activity is defined as the number of units per milligram of protein. Prior to ion exchange chromatography, it is necessary to reduce the salt concentration of the sample." @default.
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- W977996467 date "1971-01-01" @default.
- W977996467 modified "2023-09-27" @default.
- W977996467 title "[149c] Urocanase (Pseudomonas putida)" @default.
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- W977996467 doi "https://doi.org/10.1016/0076-6879(71)17011-5" @default.
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