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- W97804979 abstract "An original derivatization method for short-chain carboxylic acids (SCAs), including volatile fatty acids (VFAs), lactic and succinic acids, followed by revesed-phase (RP) HPLC with photodiode array detection has been described. Initially, all acids present in the samples were converted into sodium salts (RCOONa); then, derivatization of RCOONa was carried out with an excess of2,4-dibromoacetophenone and triethylamine. Under such conditions, all acids were converted into derivatives with chromophoric groups. Their high molar absorptivity and absorbance maximum located close to 259 ± 2 nm make them ideally suited for RP-HPLC analysis with UV detection. All derivatized acids were substantially retained on the C 18 column. The proposed procedure provides a simple and sensitive analytical tool for quantification of SCAs, especially VFAs, in biological samples. As acid derivatization is carried out at 45 + 2°C for only 60 min, the proposed procedure can be used for determination of polyunsaturated fatty acids." @default.
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- W97804979 date "2008-01-01" @default.
- W97804979 modified "2023-09-23" @default.
- W97804979 title "Efficient Procedure for Pre-Column Derivatization of Fatty Acids with Emphasis on Short-Chain Carboxylic Acids" @default.
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