Matches in SemOpenAlex for { <https://semopenalex.org/work/W978242334> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W978242334 endingPage "214" @default.
- W978242334 startingPage "202" @default.
- W978242334 abstract "This chapter describes the immobilization of the NADH and NADPH: FMN oxidoreductases and luciferase from Beneckea harveyi onto arylamine glass beads. These immobilized enzymes are individually active and also function to produce light via a coupled reaction utilizing NADH or NADPH. NADH and NADPH: FMN oxidoreductases may be obtained in a highly purified form by first separating the NADH and NADPH:FMN oxidoreductase activities as they elute from the DEAE-Sephadex A-50 column. The immobilization of an enzyme generally results in increased stability and the additional property of reusability, while retaining specificity for substrates and effectors. The availability of highly purified oxidoreductases specific for NADH or NADPH has led to the development of an immobilized enzyme system capable of continuous monitoring of reactions producing NADH or NADPH. With use the immobilized enzymes slowly lose activity, dependent upon the system and conditions of the assays." @default.
- W978242334 created "2016-06-24" @default.
- W978242334 creator A5050128275 @default.
- W978242334 creator A5059175944 @default.
- W978242334 date "1978-01-01" @default.
- W978242334 modified "2023-09-25" @default.
- W978242334 title "[23] Immobilization of bacterial luciferase and oxidoreductase and assays using immobilized enzymes" @default.
- W978242334 cites W1514743628 @default.
- W978242334 cites W1550668714 @default.
- W978242334 cites W1979978044 @default.
- W978242334 cites W2018916701 @default.
- W978242334 cites W2048074740 @default.
- W978242334 cites W206359856 @default.
- W978242334 cites W2082867002 @default.
- W978242334 cites W2086407996 @default.
- W978242334 cites W2149156626 @default.
- W978242334 doi "https://doi.org/10.1016/0076-6879(78)57025-0" @default.
- W978242334 hasPublicationYear "1978" @default.
- W978242334 type Work @default.
- W978242334 sameAs 978242334 @default.
- W978242334 citedByCount "5" @default.
- W978242334 crossrefType "book-chapter" @default.
- W978242334 hasAuthorship W978242334A5050128275 @default.
- W978242334 hasAuthorship W978242334A5059175944 @default.
- W978242334 hasConcept C104317684 @default.
- W978242334 hasConcept C111335760 @default.
- W978242334 hasConcept C163994747 @default.
- W978242334 hasConcept C181199279 @default.
- W978242334 hasConcept C185592680 @default.
- W978242334 hasConcept C197957613 @default.
- W978242334 hasConcept C2779268744 @default.
- W978242334 hasConcept C33633552 @default.
- W978242334 hasConcept C43617362 @default.
- W978242334 hasConcept C54009773 @default.
- W978242334 hasConcept C55493867 @default.
- W978242334 hasConceptScore W978242334C104317684 @default.
- W978242334 hasConceptScore W978242334C111335760 @default.
- W978242334 hasConceptScore W978242334C163994747 @default.
- W978242334 hasConceptScore W978242334C181199279 @default.
- W978242334 hasConceptScore W978242334C185592680 @default.
- W978242334 hasConceptScore W978242334C197957613 @default.
- W978242334 hasConceptScore W978242334C2779268744 @default.
- W978242334 hasConceptScore W978242334C33633552 @default.
- W978242334 hasConceptScore W978242334C43617362 @default.
- W978242334 hasConceptScore W978242334C54009773 @default.
- W978242334 hasConceptScore W978242334C55493867 @default.
- W978242334 hasLocation W9782423341 @default.
- W978242334 hasOpenAccess W978242334 @default.
- W978242334 hasPrimaryLocation W9782423341 @default.
- W978242334 hasRelatedWork W1542448467 @default.
- W978242334 hasRelatedWork W1656364975 @default.
- W978242334 hasRelatedWork W1953994264 @default.
- W978242334 hasRelatedWork W1988672938 @default.
- W978242334 hasRelatedWork W2009109153 @default.
- W978242334 hasRelatedWork W2026187888 @default.
- W978242334 hasRelatedWork W2095003531 @default.
- W978242334 hasRelatedWork W2167700458 @default.
- W978242334 hasRelatedWork W2347308429 @default.
- W978242334 hasRelatedWork W978242334 @default.
- W978242334 isParatext "false" @default.
- W978242334 isRetracted "false" @default.
- W978242334 magId "978242334" @default.
- W978242334 workType "book-chapter" @default.