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- W978300841 abstract "Publisher Summary This chapter describes the protocols for the solubilization and purification of phytoene desaturase from Phycomyces blakesleeanus. Many crude extracts, from a wide range of tissues, show phytoene desaturase activity; however, relatively few solubilization procedures have been reported, and its purification has proved to be very difficult. The name phytoene desaturase, or phytoene dehydrogenase, is given to the enzyme or enzymes, which catalyze the sequence of four didehydrogenations, which convert phytoene to phytofluene, ζ-carotene, neurosporene, and lycopene. An isomerization, probably at the phytofluene stage, from 15-cis to the all-trans configuration also occurs. The number of enzymes required for this sequence is unknown and probably varies among organisms. Genetic studies with higher plants indicate that two enzymes are necessary, but the complementation analyses of mutants of Phycomyces blakesleeanus have shown that a single gene, carB, encodes phytoene desaturase. This protein may form a tetrameric aggregate in vivo. The ion-exchange step is only applicable to phytoene desaturase preparations solubilized with a nonionic detergent." @default.
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- W978300841 date "1993-01-01" @default.
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- W978300841 title "[33] Solubilization and purification procedures for phytoene desaturase from Phycomyces blakesleeanus" @default.
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