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- W979448729 abstract "Publisher Summary This chapter discusses the applications of ligand-binding assays to ion channels and receptors expressed in Xenopus oocytes and presents some general considerations for ligand-binding assays using oocytes. Ligand-binding assays are performed by incubating intact cells, cell extracts, or purified membranes with a radiolabeled ligand for a period of time that is sufficient for equilibrium to be attained. The receptor–ligand complex is separated from the unbound ligand and the amount bound is quantified by liquid scintillation or γ counting. The chapter describes two different protocols for binding assays; cell surface receptor binding of [ 125 I]SARILE to angiotensin II receptors and 125 I-labeled α-Bungarotoxin binding to acetylcholine receptors in oocyte extracts, which is used to determine the total number of acetylcholine receptors expressed in oocytes after the injection of in vitro transcripts." @default.
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- W979448729 date "1992-01-01" @default.
- W979448729 modified "2023-09-27" @default.
- W979448729 title "[23] Ligand-binding assays in Xenopus oocytes" @default.
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- W979448729 doi "https://doi.org/10.1016/0076-6879(92)07025-j" @default.
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