Matches in SemOpenAlex for { <https://semopenalex.org/work/W9808549> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W9808549 abstract "Anti-human uracil DNA glycosylase monoclonal antibodies have been used to study the in vitro and in vivo biosynthesis of this human base excision repair enzyme. The in vitro translation of poly(A+)uracil DNA glycosylase mRNA was examined by immunoprecipitation of the (/sup 35/S)methionine-labeled translation products. As identified by sucrose density analysis, immunoreactive in vitro products of 37,000 daltons and 24,000 daltons were translated from 16S poly(A+)RNA and from 11S poly (A+)RNA, respectively. However, only the 37,000 Mr polypeptide could be detected by immunoblot analysis of crude human placental and fibroblast extracts or by immunoprecipitation of (/sup 35/S) labeled normal human cell extracts. This relative molecular weight correspond to the molecular weight observed for homogeneous human placental uracil DNA glycosylase. A placental mRNA preparation was used to construct a cDNA library in pUC8. Immunological screening identified two recombinant DNA plasmids each containing a 340 base pair insert. Northern blot analysis demonstrated that the insert hybridized to a 16S poly (A+)mRNA. These results suggest that immunoreactive uracil DNA glycosylase protein was synthesized at its enzymatically active molecular weight as the primary translation product of a 16S poly (A+)mRNA." @default.
- W9808549 created "2016-06-24" @default.
- W9808549 creator A5002956067 @default.
- W9808549 creator A5008950157 @default.
- W9808549 creator A5023943839 @default.
- W9808549 date "1987-05-01" @default.
- W9808549 modified "2023-09-23" @default.
- W9808549 title "Biosynthesis of the human DNA repair enzyme uracil DNA glycosylase" @default.
- W9808549 hasPublicationYear "1987" @default.
- W9808549 type Work @default.
- W9808549 sameAs 9808549 @default.
- W9808549 citedByCount "0" @default.
- W9808549 crossrefType "journal-article" @default.
- W9808549 hasAuthorship W9808549A5002956067 @default.
- W9808549 hasAuthorship W9808549A5008950157 @default.
- W9808549 hasAuthorship W9808549A5023943839 @default.
- W9808549 hasConcept C104317684 @default.
- W9808549 hasConcept C134935766 @default.
- W9808549 hasConcept C153911025 @default.
- W9808549 hasConcept C192396546 @default.
- W9808549 hasConcept C2778711568 @default.
- W9808549 hasConcept C2779554091 @default.
- W9808549 hasConcept C552990157 @default.
- W9808549 hasConcept C55493867 @default.
- W9808549 hasConcept C67705224 @default.
- W9808549 hasConcept C71829478 @default.
- W9808549 hasConcept C86803240 @default.
- W9808549 hasConceptScore W9808549C104317684 @default.
- W9808549 hasConceptScore W9808549C134935766 @default.
- W9808549 hasConceptScore W9808549C153911025 @default.
- W9808549 hasConceptScore W9808549C192396546 @default.
- W9808549 hasConceptScore W9808549C2778711568 @default.
- W9808549 hasConceptScore W9808549C2779554091 @default.
- W9808549 hasConceptScore W9808549C552990157 @default.
- W9808549 hasConceptScore W9808549C55493867 @default.
- W9808549 hasConceptScore W9808549C67705224 @default.
- W9808549 hasConceptScore W9808549C71829478 @default.
- W9808549 hasConceptScore W9808549C86803240 @default.
- W9808549 hasLocation W98085491 @default.
- W9808549 hasOpenAccess W9808549 @default.
- W9808549 hasPrimaryLocation W98085491 @default.
- W9808549 hasRelatedWork W1493605791 @default.
- W9808549 hasRelatedWork W1544899058 @default.
- W9808549 hasRelatedWork W1608154444 @default.
- W9808549 hasRelatedWork W1965828627 @default.
- W9808549 hasRelatedWork W1979395004 @default.
- W9808549 hasRelatedWork W1981389937 @default.
- W9808549 hasRelatedWork W1984841755 @default.
- W9808549 hasRelatedWork W1996629794 @default.
- W9808549 hasRelatedWork W1998291975 @default.
- W9808549 hasRelatedWork W2002978048 @default.
- W9808549 hasRelatedWork W2013643299 @default.
- W9808549 hasRelatedWork W2014207194 @default.
- W9808549 hasRelatedWork W2057795923 @default.
- W9808549 hasRelatedWork W2060346671 @default.
- W9808549 hasRelatedWork W2067732482 @default.
- W9808549 hasRelatedWork W2090377242 @default.
- W9808549 hasRelatedWork W2097881016 @default.
- W9808549 hasRelatedWork W2143117824 @default.
- W9808549 hasRelatedWork W2154224963 @default.
- W9808549 hasRelatedWork W2110570100 @default.
- W9808549 isParatext "false" @default.
- W9808549 isRetracted "false" @default.
- W9808549 magId "9808549" @default.
- W9808549 workType "article" @default.