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- W980926171 abstract "In this paper,specific primers of BFPA1/A2,BFPB1/B2,BFPC1/C2 were designed respectively according to the sequence of three specific fragments from AFLP markers which linked to non-acid /acid trait of peach.A 158 bp polymorphic band was amplified from the DNA of 69 F1 population of the intra-specific cross between cultivars 'Jingyu' and 'Flavortop' with the primer pair BFPB1/B2.Furthermore,the steady and specific amplification can be got with the primers designed in different location of the AFLP fragment AT-CTA199.The SCAR marker had the same segregation pattern as the original AFLPAT/CTA marker with a linkage distance of 2.99 cM and the AFLP marker was converted into SCAR marker successfully.The SCARBFPB1/B2 marker was tested in 60 F2 progeny of 'Okubo' and 'Xingjin' nectarine,and the results showed that the accordance ratio of genotype and phenotype was 96.7%.The SCAR marker was a co-dominant marker and could distinguish the heterozygous and homozygous.Polymorphism disappeared in parents and progeny with specific primers BFPA1/A2 and BFPC1/C2." @default.
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- W980926171 date "2009-01-01" @default.
- W980926171 modified "2023-09-23" @default.
- W980926171 title "Development and validation of SCAR markers linked to the non-acid/acid trait of fruit in peach." @default.
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