Matches in SemOpenAlex for { <https://semopenalex.org/work/W98203251> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W98203251 abstract "An efficient method for producing transgenic eggplant (Solanum melongena L.) via Agrobacterium tumefaciens-mediated genetic transformation using gus reporter gene was developed. Leaf explants were transformed by co-cultivation with Agrobacterium tumefaciens strain LBA4404 harbouring a binary vector pCAMBIA 1301 carrying the reporter gene beta-glucuronidase intron (GUS-INT) and the marker gene hygromycin phosphotransferase (hpt). Transgenic calli induced in media containing 1 mg I-I indole-3-acetic acid (IAA) and 3 mg I-I benzyladenine (BA) as well as 1 mg rl indole-3-acetic acid (IAA) and 3 mg rl kinetin (Kn), subcultured 0.1 mg I-I thidiazuron (TDZ), 3.0 mg I-I BA, produced shoot buds. Direct shoot buds were also induced in 3 mg I-I BA and 0.5 mg I-I TDZ containing medium. The putative transgenic shoot buds elongated on basal medium aud rooted efficiently on sterilised soil irrigated with water. Histochemical GUS assay and polymerase chain reaction analysis of transgenic plants confirmed the presence of the GUS and hpt genes, respectively. Integration of T-DNA into the genome of putative transgenics was further confirmed by Southern blot analysis." @default.
- W98203251 created "2016-06-24" @default.
- W98203251 creator A5005203729 @default.
- W98203251 creator A5012622592 @default.
- W98203251 creator A5013442089 @default.
- W98203251 creator A5020033569 @default.
- W98203251 creator A5047048024 @default.
- W98203251 date "2005-01-01" @default.
- W98203251 modified "2023-09-24" @default.
- W98203251 title "Transformation and Regeneration of Solanum melongena using Agrobacterium tumeJaciens" @default.
- W98203251 cites W1562393320 @default.
- W98203251 cites W1969020164 @default.
- W98203251 cites W2016000860 @default.
- W98203251 cites W2021539673 @default.
- W98203251 cites W2024556021 @default.
- W98203251 cites W2103766097 @default.
- W98203251 cites W2157305530 @default.
- W98203251 cites W245950490 @default.
- W98203251 hasPublicationYear "2005" @default.
- W98203251 type Work @default.
- W98203251 sameAs 98203251 @default.
- W98203251 citedByCount "1" @default.
- W98203251 countsByYear W982032512014 @default.
- W98203251 crossrefType "journal-article" @default.
- W98203251 hasAuthorship W98203251A5005203729 @default.
- W98203251 hasAuthorship W98203251A5012622592 @default.
- W98203251 hasAuthorship W98203251A5013442089 @default.
- W98203251 hasAuthorship W98203251A5020033569 @default.
- W98203251 hasAuthorship W98203251A5047048024 @default.
- W98203251 hasConcept C102230213 @default.
- W98203251 hasConcept C104317684 @default.
- W98203251 hasConcept C104320060 @default.
- W98203251 hasConcept C122172344 @default.
- W98203251 hasConcept C150194340 @default.
- W98203251 hasConcept C153911025 @default.
- W98203251 hasConcept C157890192 @default.
- W98203251 hasConcept C161733203 @default.
- W98203251 hasConcept C202751555 @default.
- W98203251 hasConcept C204241405 @default.
- W98203251 hasConcept C21410773 @default.
- W98203251 hasConcept C2776162087 @default.
- W98203251 hasConcept C2777322866 @default.
- W98203251 hasConcept C2779656422 @default.
- W98203251 hasConcept C2781083041 @default.
- W98203251 hasConcept C55493867 @default.
- W98203251 hasConcept C59822182 @default.
- W98203251 hasConcept C86803240 @default.
- W98203251 hasConceptScore W98203251C102230213 @default.
- W98203251 hasConceptScore W98203251C104317684 @default.
- W98203251 hasConceptScore W98203251C104320060 @default.
- W98203251 hasConceptScore W98203251C122172344 @default.
- W98203251 hasConceptScore W98203251C150194340 @default.
- W98203251 hasConceptScore W98203251C153911025 @default.
- W98203251 hasConceptScore W98203251C157890192 @default.
- W98203251 hasConceptScore W98203251C161733203 @default.
- W98203251 hasConceptScore W98203251C202751555 @default.
- W98203251 hasConceptScore W98203251C204241405 @default.
- W98203251 hasConceptScore W98203251C21410773 @default.
- W98203251 hasConceptScore W98203251C2776162087 @default.
- W98203251 hasConceptScore W98203251C2777322866 @default.
- W98203251 hasConceptScore W98203251C2779656422 @default.
- W98203251 hasConceptScore W98203251C2781083041 @default.
- W98203251 hasConceptScore W98203251C55493867 @default.
- W98203251 hasConceptScore W98203251C59822182 @default.
- W98203251 hasConceptScore W98203251C86803240 @default.
- W98203251 hasLocation W982032511 @default.
- W98203251 hasOpenAccess W98203251 @default.
- W98203251 hasPrimaryLocation W982032511 @default.
- W98203251 hasRelatedWork W1499184654 @default.
- W98203251 hasRelatedWork W1562393320 @default.
- W98203251 hasRelatedWork W1574233575 @default.
- W98203251 hasRelatedWork W2021068148 @default.
- W98203251 hasRelatedWork W2048633773 @default.
- W98203251 hasRelatedWork W2059815761 @default.
- W98203251 hasRelatedWork W2072426160 @default.
- W98203251 hasRelatedWork W2154195722 @default.
- W98203251 hasRelatedWork W2191189621 @default.
- W98203251 hasRelatedWork W2467982296 @default.
- W98203251 hasRelatedWork W2591546368 @default.
- W98203251 hasRelatedWork W3001368088 @default.
- W98203251 hasRelatedWork W3009591445 @default.
- W98203251 hasRelatedWork W3039284415 @default.
- W98203251 hasRelatedWork W3144694258 @default.
- W98203251 hasRelatedWork W91716892 @default.
- W98203251 hasRelatedWork W2181962577 @default.
- W98203251 hasRelatedWork W2182688082 @default.
- W98203251 hasRelatedWork W2341786873 @default.
- W98203251 hasRelatedWork W3033974903 @default.
- W98203251 isParatext "false" @default.
- W98203251 isRetracted "false" @default.
- W98203251 magId "98203251" @default.
- W98203251 workType "article" @default.