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- W982342608 abstract "Publisher Summary This chapter discusses the isolation of astrocytes and Schwann cells for culture. Astrocytes can be obtained in high yield and high purity (over 95%) if some special features are taken into consideration. First, the developmental stage at which astrocytes are isolated is of prime importance. In rats, the peak genesis of astrocytes spans a period between 4 days prenatal (or 17 days gestation) and 1 day postnatal, a time following neurogenesis but preceding the development of oligodendroglia. Therefore, one good source of astrocytes is fetal rat brain a few days before birth. In laboratory, 18-day-old fetal rats are routinely used. Next, astrocytes attach rapidly to plastic or glass surfaces without the need of collagen or polycation coating. The chapter describes the detailed procedure for isolation of astrocytes from prenatal rat brains. It also highlights that it is advisable for a beginner to estimate the purity of the isolated astrocytes by immunostaining for glial fibrillary acidic protein (GFAP), using an FITC-conjugated secondary antibody." @default.
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- W982342608 date "1990-01-01" @default.
- W982342608 modified "2023-09-28" @default.
- W982342608 title "Isolation of Astrocytes and Schwann Cells for Culture" @default.
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- W982342608 doi "https://doi.org/10.1016/b978-0-12-185254-2.50008-5" @default.
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