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- W983439840 abstract "This chapter discusses the determination of 2,3- cis -polyol dehydrogenase. Enzyme is assayed by determining the increase in absorption at 340 mμ because of the formation of reduced diphosphopyridine nucleotide (DPNH). A unit of activity is defined as that amount of enzyme required to form 1 micromole of DPNH per minute. Specific activity is defined as the number of units per milligram of protein; protein is measured with bovine serum albumin as a standard. The enzyme is obtained from a strain of Aerobacter aerogenes which had been isolated from an enrichment culture with erythritol as sole carbon source. Cells are harvested with a Sharples centrifuge after 24 hours of growth at room temperature, washed with 5 volumes of saline, and stored at –15°. As storage under these conditions results in drastic loss of enzyme activity within 1 week, it is advisable that the cells be used shortly after harvesting. Polyols of 3 to 5 carbons having cis hydroxyl groups at C-2 and C-3 are active." @default.
- W983439840 created "2016-06-24" @default.
- W983439840 creator A5000435529 @default.
- W983439840 date "1966-01-01" @default.
- W983439840 modified "2023-09-27" @default.
- W983439840 title "[38] 2,3-cis-Polyol dehydrogenase" @default.
- W983439840 cites W1775749144 @default.
- W983439840 doi "https://doi.org/10.1016/0076-6879(66)09045-1" @default.
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