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- W984161983 abstract "Publisher Summary This chapter describes the measurement of kinetic rate constants for the binding of 1α,25-dihydroxyvitamin D 3 to its chick intestinal mucosa receptor, using a hydroxyapatite batch assay. 1α,25-dihydroxyvitamin D 3 [1α,25-(OH) 2 D 3 ] is believed to act as a classical steroid hormone. It has been demonstrated that there are specific cytoplasmic and chromatin-extractable receptors for this steroid in chick intestine and chick parathyroid glands. In addition, cytoplasmic receptors for lα,25-(OH) 2 D 3 have been demonstrated in rat intestine and chick and rat calvaria. The lack of a convenient assay system for these macromolecular complexes and their relative instability has hindered their detailed characterization. In this study, a hydroxyapatite batch assay has been adapted for the quantitation of 1α,25-(OH) 2 D 3 receptor complex from chick intestinal mucosa cytosol. This has provided the first assay for this system, which is facile enough to be used to study the kinetics of the binding processes. This chapter describes the assay procedure and its application to the measurement of association and dissociation rate constants for the chick intestinal mucosa lα,25-(OH) 2 D 3 receptor." @default.
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- W984161983 date "1980-01-01" @default.
- W984161983 modified "2023-09-26" @default.
- W984161983 title "[58] Measurement of kinetic rate constants for the binding of 1α,25-dihydroxyvitamin D3 to its chick intestinal mucosa receptor using a hydroxyapatite batch assay" @default.
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- W984161983 doi "https://doi.org/10.1016/s0076-6879(80)67060-8" @default.
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