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- W984705150 abstract "Glycoprotein gB was the major protein to determine infection and immunogenicity of infectious Laryngotracheitis virus(ILTV).The gB glycoprotein gene of ILTV isolates were amplified by polymerase chain reaction(PCR) and proved to be gB gene by sequencing.The E.coli-mycobacterium expression shuttle plasmid pR-α-gB was constructed by inserting the gB gene to the pRR3 with human mycobacterium tuberculosis HSP70 promoter and α signal peptide.Then the plasmid pR-α-gB was introduced into mycobacterium bovis BCG by electroporation to construct a recombinate strain rBCG-gB.The gB protein could be highly expressed in M.smegmatis mc2 155 when induced by heating and was detected by ELISA and Western-blot assays using monoclonal antibody against gB glycoprotein of ILTV.3 week old SPF chinken were subcutaneously immunized twice at week 0 and 3 with 106 cfu rBCG-gB then the level of lymphocytes index(SI),macrophage activity,cytokine IL-2 and IFN-γ in serum was measured respectively.These results indicated that the recombinant strain rBCG-gB designed as BCG vector vaccine would be able to induce stronger immune response in SPF chicken than in control,vector and BCG groups.6 week-old SPF chicken were subcutaneously immunized with 106 cfu rBCG-gB and each chick was immunized three times at 3 week intervals with the same antigen used for the primary immunization.The protective immunity of rBCG-gB was identified in vaccinated chickens.Results from the protection test were evaluated the two immunizations with rBCG-gB could provide protection for chickens from the challenge with virulent ILTV Heb01strain.Haemagglutination inhibition titers were also increased in chickens immunized with the expressed rBCG-gB,and significantly higher titers were detected after challenge.These data indicate that the rBCG-gB could be used as candidate of a live vector vaccine for ILTV." @default.
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- W984705150 date "2009-01-01" @default.
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- W984705150 title "Construction of recombinant BCG bearing gB glycoprotein gene of ILTV and study on its immunogenicity on chickens." @default.
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