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- W985138313 abstract "Interactions of viruses with the host cell comprise their adsorption to the cell surface (in most cases via specific receptors), uptake and transport in the cytoplasm, followed by uncoating and release of the genome, nucleic acid replication, and finally synthesis of virus proteins leading to the assembly of new virus progeny. All these stages in the virus/cell interaction can be monitored and studied with the electron microscope, but a range of different preparatory methods are required. This chapter discusses the different preparatory methods, including ultrathin sectioning, scanning electron microscopy, replicas of cell surfaces or cell interior, and a series of more sophisticated procedures such as autoradiography, low-temperature electron microscopy of frozen sections, cytochemistry and immunolabeling. The chapter discusses immuno-electron microscopy (IEM) in virology. IEM combines antibody specificity in detecting antigenic determinants and the high spatial resolution of electron microscopy in a most favourable way. In virology, IEM can either be applied to search for virus constituents in infected cells." @default.
- W985138313 created "2016-06-24" @default.
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- W985138313 date "1987-01-01" @default.
- W985138313 modified "2023-09-27" @default.
- W985138313 title "Chapter 2B Electron microscopy: methods for studies of virus particles and virus-infected cells" @default.
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- W985138313 doi "https://doi.org/10.1016/s0168-7069(08)70083-1" @default.
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