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- W985272040 abstract "Under appropriate conditions, the retina can be sustained in vitro for several hours with preservation of normal cellular architecture and metabolic and electrophysiological competence. The vertebrate retina can be thought of as an “instant tissue slice,” being only 0.1 mm–0.3 mm in thickness. Small molecules can diffuse readily in and out of the tissue. With judicious choice of radiolabeled precursors and pulse-chase protocols, one can label intracellular substrate pools of retinal cells to relatively high specific activities. The isolated retina is well suited for parallel electrophysiological studies to evaluate the consequences of variations in extracellular composition and ambient conditions. This chapter describes the specifications and practical considerations of in vitro systems, with particular emphasis on amphibian retinas. The specific examples given in the chapter illustrate the use of isolated retinas for studying the biosynthesis and metabolism of molecules employed in the assembly of rod outer segment (ROS) membranes. Compared with that of total retina, the simplicity of the ROS membrane protein composition is easily appreciated from the Coomassie blue–stained gel." @default.
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- W985272040 date "1993-01-01" @default.
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- W985272040 title "In Vitro Biosynthetic Studies with Isolated Vertebrate Retinas" @default.
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- W985272040 doi "https://doi.org/10.1016/b978-0-12-185279-5.50011-x" @default.
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