Matches in SemOpenAlex for { <https://semopenalex.org/work/W98573403> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W98573403 endingPage "72" @default.
- W98573403 startingPage "66" @default.
- W98573403 abstract "cAMP plays a critical role in intracellular signaling pathways that regulate proliferation or differentiation. The cAMP binding protein assay, using a naturally derived cAMP binding protein, is one of the most widely used methods for cAMP determination. The major steps of this binding assay include purification of the binding protein, cAMP extraction from samples, and quantification of the cAMP Most purification methods of the cAMP binding protein were published before 1975, and many of the materials and methods are outdated. Here we describe an updated method of purification of cAMP binding protein from bovine skeletal muscle with the advantages of simplicity, low cost, and high yield The isolation procedures can be completed in two days using commercially available materials and equipment. The cAMP binding properties of the isolated protein can be utilizedfor more than two years. Binding protein isolatedfrom 1 kg bovine muscle is sufficientfor at least 3 x10(4) assay tubes. Furthemore, we describe the techniques of cAMP extraction and quantification that have been used successfully in studying parathyroid hormone signaling as an example of a G protein-linked seven transmembrane domain receptor that signals through the protein kinase A pathway." @default.
- W98573403 created "2016-06-24" @default.
- W98573403 creator A5010672369 @default.
- W98573403 creator A5017436055 @default.
- W98573403 creator A5040201467 @default.
- W98573403 date "2002-07-01" @default.
- W98573403 modified "2023-09-25" @default.
- W98573403 title "cAMP Binding Protein Assay for Widespread Use in Cell Signaling Studies" @default.
- W98573403 cites W1576399086 @default.
- W98573403 cites W1985148410 @default.
- W98573403 cites W2044227713 @default.
- W98573403 cites W2047865806 @default.
- W98573403 cites W2055466090 @default.
- W98573403 cites W69093701 @default.
- W98573403 doi "https://doi.org/10.2144/02331st03" @default.
- W98573403 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12139259" @default.
- W98573403 hasPublicationYear "2002" @default.
- W98573403 type Work @default.
- W98573403 sameAs 98573403 @default.
- W98573403 citedByCount "7" @default.
- W98573403 countsByYear W985734032015 @default.
- W98573403 countsByYear W985734032019 @default.
- W98573403 countsByYear W985734032020 @default.
- W98573403 crossrefType "journal-article" @default.
- W98573403 hasAuthorship W98573403A5010672369 @default.
- W98573403 hasAuthorship W98573403A5017436055 @default.
- W98573403 hasAuthorship W98573403A5040201467 @default.
- W98573403 hasBestOaLocation W985734031 @default.
- W98573403 hasConcept C104317684 @default.
- W98573403 hasConcept C11960822 @default.
- W98573403 hasConcept C1629964 @default.
- W98573403 hasConcept C170493617 @default.
- W98573403 hasConcept C185592680 @default.
- W98573403 hasConcept C199172495 @default.
- W98573403 hasConcept C51639874 @default.
- W98573403 hasConcept C553089730 @default.
- W98573403 hasConcept C55493867 @default.
- W98573403 hasConcept C62478195 @default.
- W98573403 hasConcept C85196483 @default.
- W98573403 hasConcept C86339819 @default.
- W98573403 hasConcept C86803240 @default.
- W98573403 hasConcept C8985478 @default.
- W98573403 hasConcept C95444343 @default.
- W98573403 hasConcept C97029542 @default.
- W98573403 hasConceptScore W98573403C104317684 @default.
- W98573403 hasConceptScore W98573403C11960822 @default.
- W98573403 hasConceptScore W98573403C1629964 @default.
- W98573403 hasConceptScore W98573403C170493617 @default.
- W98573403 hasConceptScore W98573403C185592680 @default.
- W98573403 hasConceptScore W98573403C199172495 @default.
- W98573403 hasConceptScore W98573403C51639874 @default.
- W98573403 hasConceptScore W98573403C553089730 @default.
- W98573403 hasConceptScore W98573403C55493867 @default.
- W98573403 hasConceptScore W98573403C62478195 @default.
- W98573403 hasConceptScore W98573403C85196483 @default.
- W98573403 hasConceptScore W98573403C86339819 @default.
- W98573403 hasConceptScore W98573403C86803240 @default.
- W98573403 hasConceptScore W98573403C8985478 @default.
- W98573403 hasConceptScore W98573403C95444343 @default.
- W98573403 hasConceptScore W98573403C97029542 @default.
- W98573403 hasIssue "1" @default.
- W98573403 hasLocation W985734031 @default.
- W98573403 hasLocation W985734032 @default.
- W98573403 hasLocation W985734033 @default.
- W98573403 hasOpenAccess W98573403 @default.
- W98573403 hasPrimaryLocation W985734031 @default.
- W98573403 hasRelatedWork W1997721198 @default.
- W98573403 hasRelatedWork W2028023117 @default.
- W98573403 hasRelatedWork W2060357870 @default.
- W98573403 hasRelatedWork W2065612361 @default.
- W98573403 hasRelatedWork W2079583561 @default.
- W98573403 hasRelatedWork W2089807640 @default.
- W98573403 hasRelatedWork W2145616182 @default.
- W98573403 hasRelatedWork W3109772286 @default.
- W98573403 hasRelatedWork W3156080906 @default.
- W98573403 hasRelatedWork W98573403 @default.
- W98573403 hasVolume "33" @default.
- W98573403 isParatext "false" @default.
- W98573403 isRetracted "false" @default.
- W98573403 magId "98573403" @default.
- W98573403 workType "article" @default.