Matches in SemOpenAlex for { <https://semopenalex.org/work/W98587679> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W98587679 endingPage "52" @default.
- W98587679 startingPage "247" @default.
- W98587679 abstract "Anti-neutrophil cytoplasmic antibodies (ANCA) are a family of autoantibodies which react with components of phagocytic cells, and are associated with vasculitis and other idiopathic inflammatory disorders. However, the antigenic targets of many of these autoantibodies have not been defined yet. In this study, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF) were evaluated for characterising the antigenic specificity of unidentified ANCA. The uncharacterised sera included those from patients with ulcerative colitis (n = 21), Crohn's disease (n = 5), cystic fibrosis (n = 16) and sarcoidosis (n = 2). In addition, sera from patients with antibodies to the phagocytic enzymes proteinase 3 (PR3) (n = 11) and myeloperoxidase (MPO) (n = 5) were also included. The sub-cellular localisation of antigens was determined by testing sera against crude neutrophil extract and sub-cellular fractions consisting of azurophilic granules, specific granules and cytosolic, fractions using enzyme-linked immunosorbent assays (ELISAs). All sera reacted with the crude and azurophilic granule extracts. The native system of IEF followed by capillary immunoblotting successfully detected anti-PR3 and anti-MPO in azurophilic granule extracts. In contrast, SDS-PAGE Western blotting failed to detect any reactivity, either to PR3 or MPO, in the crude extract or azurophilic granule extract. However, the antibody specificity of patient sera with uncharacterised autoantibodies could not be detected by IEF/capillary immunoblotting or SDS-PAGE. This study showed that the sub-cellular azurophilic granules are the antigenic target of a variety of uncharacterised ANCA. It also showed that IEF characterised both anti-PR3 and anti-MPO but failed to detect other forms of ANCA. In contrast, the majority of common ANCA were not detected by SDS-PAGE." @default.
- W98587679 created "2016-06-24" @default.
- W98587679 creator A5013189412 @default.
- W98587679 creator A5022290849 @default.
- W98587679 creator A5064821149 @default.
- W98587679 date "1998-12-01" @default.
- W98587679 modified "2023-09-23" @default.
- W98587679 title "Characterisation of anti-neutrophil cytoplasmic antibody target antigens using electrophoresis and western blotting techniques." @default.
- W98587679 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10436539" @default.
- W98587679 hasPublicationYear "1998" @default.
- W98587679 type Work @default.
- W98587679 sameAs 98587679 @default.
- W98587679 citedByCount "2" @default.
- W98587679 crossrefType "journal-article" @default.
- W98587679 hasAuthorship W98587679A5013189412 @default.
- W98587679 hasAuthorship W98587679A5022290849 @default.
- W98587679 hasAuthorship W98587679A5064821149 @default.
- W98587679 hasConcept C104317684 @default.
- W98587679 hasConcept C116084860 @default.
- W98587679 hasConcept C147483822 @default.
- W98587679 hasConcept C153911025 @default.
- W98587679 hasConcept C159654299 @default.
- W98587679 hasConcept C163764329 @default.
- W98587679 hasConcept C174106267 @default.
- W98587679 hasConcept C181199279 @default.
- W98587679 hasConcept C182220744 @default.
- W98587679 hasConcept C185592680 @default.
- W98587679 hasConcept C203014093 @default.
- W98587679 hasConcept C203565725 @default.
- W98587679 hasConcept C21194631 @default.
- W98587679 hasConcept C2776914184 @default.
- W98587679 hasConcept C2777132095 @default.
- W98587679 hasConcept C2779170986 @default.
- W98587679 hasConcept C50476208 @default.
- W98587679 hasConcept C55493867 @default.
- W98587679 hasConcept C85265743 @default.
- W98587679 hasConcept C86803240 @default.
- W98587679 hasConceptScore W98587679C104317684 @default.
- W98587679 hasConceptScore W98587679C116084860 @default.
- W98587679 hasConceptScore W98587679C147483822 @default.
- W98587679 hasConceptScore W98587679C153911025 @default.
- W98587679 hasConceptScore W98587679C159654299 @default.
- W98587679 hasConceptScore W98587679C163764329 @default.
- W98587679 hasConceptScore W98587679C174106267 @default.
- W98587679 hasConceptScore W98587679C181199279 @default.
- W98587679 hasConceptScore W98587679C182220744 @default.
- W98587679 hasConceptScore W98587679C185592680 @default.
- W98587679 hasConceptScore W98587679C203014093 @default.
- W98587679 hasConceptScore W98587679C203565725 @default.
- W98587679 hasConceptScore W98587679C21194631 @default.
- W98587679 hasConceptScore W98587679C2776914184 @default.
- W98587679 hasConceptScore W98587679C2777132095 @default.
- W98587679 hasConceptScore W98587679C2779170986 @default.
- W98587679 hasConceptScore W98587679C50476208 @default.
- W98587679 hasConceptScore W98587679C55493867 @default.
- W98587679 hasConceptScore W98587679C85265743 @default.
- W98587679 hasConceptScore W98587679C86803240 @default.
- W98587679 hasIssue "4" @default.
- W98587679 hasLocation W985876791 @default.
- W98587679 hasOpenAccess W98587679 @default.
- W98587679 hasPrimaryLocation W985876791 @default.
- W98587679 hasRelatedWork W1510738661 @default.
- W98587679 hasRelatedWork W1976784572 @default.
- W98587679 hasRelatedWork W1978422915 @default.
- W98587679 hasRelatedWork W2059794164 @default.
- W98587679 hasRelatedWork W2111358034 @default.
- W98587679 hasRelatedWork W2145088285 @default.
- W98587679 hasRelatedWork W2147533022 @default.
- W98587679 hasRelatedWork W2151252177 @default.
- W98587679 hasRelatedWork W775029226 @default.
- W98587679 hasRelatedWork W98587679 @default.
- W98587679 hasVolume "55" @default.
- W98587679 isParatext "false" @default.
- W98587679 isRetracted "false" @default.
- W98587679 magId "98587679" @default.
- W98587679 workType "article" @default.