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- W98650897 abstract "Both glucocorticoids and glucagon (cAMP) were essential for stimulating transcription of the serine dehydratase (SDH) gene in primary cultures of adult rat hepatocytes. We cloned the gene encoding the rat SDH gene, and fused 2 Kb of its 5’-flanking sequence with the chloramphenicol acetyltransferase gene. Primary cultures of adult rat hepatocytes were transfected with the fusion gene. Unlike expression of the endogenous SDH gene, that of the fusion gene was stimulated by either dexamethasone or glucagon alone, and the two hormones did not show synergistic effects. These results suggest that the 2 kb 5’-flanking sequence of the SDH gene contains glucocorticoid- and cAMP-responsive elements, and these elements have become accessible independently to enhance transient expression of the fusion gene, but not expression of the chromosomal SDH gene." @default.
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- W98650897 date "1991-01-01" @default.
- W98650897 modified "2023-09-25" @default.
- W98650897 title "Characterization and Expression of the Serine Dehydratase Gene: Regulation by Glucocorticoids and Cyclic Amp" @default.
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- W98650897 doi "https://doi.org/10.1016/b978-0-08-040820-0.50111-4" @default.
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