Matches in SemOpenAlex for { <https://semopenalex.org/work/W986832418> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W986832418 endingPage "351" @default.
- W986832418 startingPage "349" @default.
- W986832418 abstract "This chapter describes an assay method and the purification procedure for fructose-l,6-bisphosphatase from snake muscle (Zaocys dhumnades). The assay is based on the estimation of the inorganic phosphate formed by the hydrolysis of fructose 1,6-bisphosphate (Fru-P2) using a modification of the method of Fiske and Subbarow. The purification process involves extraction, heat treatment, ammonium sulfate fractionation, and phosphocellulose absorption (ammonium sulfate fraction). The purified enzyme shows a single band in sodium dodecyl sulfate-gel electrophoresis corresponding to a subunit weight of 35,000 and a molecular weight of 140,000 is estimated by gel filtration. The maximum activity is in the neutral range and the ratio of activity measured at pH 7.5 to that measured at pH 9.2 is in excess of 2.5. Snake muscle Fru-P2ase shows an absolute requirement for divalent metal cations, which can be satisfied by Mg2+, Mn2+, or Zn2+. In the absence of KCl, the pH optimum for the snake muscle enzyme is at pH 6.6; in the presence of 0.4 M KC1, the optimum shifts to pH 7.0." @default.
- W986832418 created "2016-06-24" @default.
- W986832418 creator A5072511448 @default.
- W986832418 creator A5078022081 @default.
- W986832418 creator A5090464582 @default.
- W986832418 date "1982-01-01" @default.
- W986832418 modified "2023-10-02" @default.
- W986832418 title "[58] Fructose-1,6-bisphosphatase from snake muscle (Zaocys dhumnades)" @default.
- W986832418 cites W1775749144 @default.
- W986832418 cites W2018509223 @default.
- W986832418 cites W2086351654 @default.
- W986832418 cites W2153435210 @default.
- W986832418 doi "https://doi.org/10.1016/s0076-6879(82)90154-9" @default.
- W986832418 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6296609" @default.
- W986832418 hasPublicationYear "1982" @default.
- W986832418 type Work @default.
- W986832418 sameAs 986832418 @default.
- W986832418 citedByCount "14" @default.
- W986832418 crossrefType "book-chapter" @default.
- W986832418 hasAuthorship W986832418A5072511448 @default.
- W986832418 hasAuthorship W986832418A5078022081 @default.
- W986832418 hasAuthorship W986832418A5090464582 @default.
- W986832418 hasConcept C178790620 @default.
- W986832418 hasConcept C181199279 @default.
- W986832418 hasConcept C185592680 @default.
- W986832418 hasConcept C189909181 @default.
- W986832418 hasConcept C193363816 @default.
- W986832418 hasConcept C2776970464 @default.
- W986832418 hasConcept C2778913249 @default.
- W986832418 hasConcept C2779870022 @default.
- W986832418 hasConcept C2780925461 @default.
- W986832418 hasConcept C43617362 @default.
- W986832418 hasConcept C537181965 @default.
- W986832418 hasConcept C55493867 @default.
- W986832418 hasConcept C74884574 @default.
- W986832418 hasConcept C94412978 @default.
- W986832418 hasConcept C97428945 @default.
- W986832418 hasConceptScore W986832418C178790620 @default.
- W986832418 hasConceptScore W986832418C181199279 @default.
- W986832418 hasConceptScore W986832418C185592680 @default.
- W986832418 hasConceptScore W986832418C189909181 @default.
- W986832418 hasConceptScore W986832418C193363816 @default.
- W986832418 hasConceptScore W986832418C2776970464 @default.
- W986832418 hasConceptScore W986832418C2778913249 @default.
- W986832418 hasConceptScore W986832418C2779870022 @default.
- W986832418 hasConceptScore W986832418C2780925461 @default.
- W986832418 hasConceptScore W986832418C43617362 @default.
- W986832418 hasConceptScore W986832418C537181965 @default.
- W986832418 hasConceptScore W986832418C55493867 @default.
- W986832418 hasConceptScore W986832418C74884574 @default.
- W986832418 hasConceptScore W986832418C94412978 @default.
- W986832418 hasConceptScore W986832418C97428945 @default.
- W986832418 hasLocation W9868324181 @default.
- W986832418 hasLocation W9868324182 @default.
- W986832418 hasOpenAccess W986832418 @default.
- W986832418 hasPrimaryLocation W9868324181 @default.
- W986832418 hasRelatedWork W1520443578 @default.
- W986832418 hasRelatedWork W1601079487 @default.
- W986832418 hasRelatedWork W1965142764 @default.
- W986832418 hasRelatedWork W1966862125 @default.
- W986832418 hasRelatedWork W1988905094 @default.
- W986832418 hasRelatedWork W2004231513 @default.
- W986832418 hasRelatedWork W2048642571 @default.
- W986832418 hasRelatedWork W2074675460 @default.
- W986832418 hasRelatedWork W2076636492 @default.
- W986832418 hasRelatedWork W2491525353 @default.
- W986832418 isParatext "false" @default.
- W986832418 isRetracted "false" @default.
- W986832418 magId "986832418" @default.
- W986832418 workType "book-chapter" @default.