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• W988815830 abstract "The cell envelope of mycobacteria, i.e., the compounds that surround the cytoplasm and protect the micro-organisms from their environment, is important for the bacterial physiology because inhibition of the production of some of its constituents, e.g., mycolic acids and arabinogalactan, kills the cells. It is also this structure that controls the transfer of materials into and out of the mycobacterium. The wall possesses a fundamental, covalently linked “cell-wall skeleton” (CWS) associated with a great variety of noncovalently linked substances, of which a majority are lipids and glycolipids. Isolated plasma membranes are typically obtained by breaking the cells by mechanical stress, e.g., sonication or shearing in the French pressure cell, followed by fractionation using differential centrifugation or density gradients. The wall of mycobacteria consists of a covalently linked CWS, and an abundant variety of wall-associated lipids and a few polypeptides. That the outermost layer of the mycobacterial cell envelope is partly polysaccharide in nature could be deduced from the staining of electron-transparent zone (ETZ) with peroxidase-conjugated concanavalin A and from old data showing that mycobacterial culture filtrates, notably the preparation called tuberculin, contain several polysaccharides. The polysaccharide/protein matrix in pathogens would also serve as a defense mechanism by facilitating survival in the host. The capsule and its constituents can also act by actively modifying the behavior of host cells in some way beneficial to the bacterium, as recently shown for the glucan from Mycobacterium tuberculosis." @default.
• W988815830 created "2016-06-24" @default.
• W988815830 creator A5041220789 @default.
• W988815830 date "2014-04-30" @default.
• W988815830 modified "2023-09-30" @default.
• W988815830 title "The Global Architecture of the Mycobacterial Cell Envelope" @default.
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• W988815830 doi "https://doi.org/10.1128/9781555815783.ch1" @default.
• W988815830 hasPublicationYear "2014" @default.
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