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• W989088954 abstract "This chapter discusses the serial cultivation of the normal human epidermal keratinocytes. Fibroblasts can be grown from almost any explanted human tissue and can be serially passaged and even cloned from low-density platings. Keratinocyte colonies are initiated by plating a single-cell suspension of keratinocytes with 3T3 feeder cells in the usual way such that less than 300 colonies arise. Newborn foreskin is a convenient source of human keratinocytes possessing a high-proliferative capacity in culture. Trypsin alone is sufficient to completely disaggregate this tissue to single cells with a yield of about 3 million cells per square centimeter of epidermal surface. The keratinocytes attach directly to the culture vessel surface and push away the 3T3 feeder cells as the colonies expand. In secondary and subsequent cultures, however, keratinocytes do not assume normal epithelioid morphology during the early stage of colony initiation in the absence of hydrocortisone, although colony-forming efficiency is unaffected. Cells growing in the absence of hydrocortisone eventually organize into normal colony morphology by the time colonies have reached a size of about 100 cells. The expression of tissue-specific structure and function by cultured keratinocytes is discussed in the chapter." @default.
• W989088954 created "2016-06-24" @default.
• W989088954 creator A5085721535 @default.
• W989088954 date "1980-01-01" @default.
• W989088954 modified "2023-10-11" @default.
• W989088954 title "Chapter 15 Serial Cultivation of Normal Human Epidermal Keratinocytes" @default.
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• W989088954 doi "https://doi.org/10.1016/s0091-679x(08)60769-4" @default.
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