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- W989548102 abstract "This chapter describes the methods used for the generation of perforated cells using the nitrocellulose filter procedure, with the aim of providing a general approach that can be adapted to different cell types. The chapter presents results on the characterization of the perforated cells and their use in the reconstitution of membrane transport. The different systems provided different results concerning the requirements for membrane transport. Madin-Darby canine kidney (MDCK) and BHK cells grown on glass provided very similar results. In both cases, transport from the endoplasmic reticulum (ER) to the Golgi and from the trans-Golgi network (TGN) to the plasma membrane proceeded efficiently. The main difference between the two systems was that in BHK cells transport was dependent on glucose, whereas in MDCK cells transport was dependent on adenosine triphosphate (ATP). This difference may reflect a difference in the metabolism in the two cells." @default.
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- W989548102 date "1989-01-01" @default.
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- W989548102 title "Chapter 6 Perforated Cells for Studying Intracellular Membrane Transport" @default.
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- W989548102 doi "https://doi.org/10.1016/s0091-679x(08)61604-0" @default.
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