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- W989801219 abstract "A mouse hybrid cell line producing an antibody to human nervous system-specific γγ enolase has been isolated by fusions between γγ-immunized mouse spleen cells and mouse myeloma cells (P3-NS-1/1-Ag4-1), followed by a screening procedure with an enzyme immunoassay. This particular cell line (E1-G3) has secreted the antibody bearing γ2a/x immunoglobulin chains. Specificity of the E1-G3 antibody was tested by immunoprecipitation of enolase activities with anti-mouse IgG, and by use of enzyme immunoassay systems for enolase isozymes which consisted of polyclonal rabbit antibodies. The E1-G3 antibody was found to be specific for the γ subunit of enolase, showing reactivities with human γγ and αγ enolases, and also with rat γγ enolase. However, the monoclonal antibody did not cross-react with the α or β subunit of human enolase." @default.
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- W989801219 date "1983-01-01" @default.
- W989801219 modified "2023-09-23" @default.
- W989801219 title "Production and Characterization of a Monoclonal Antibody to Human Nervous System-Specific γγ Enolase" @default.
- W989801219 doi "https://doi.org/10.1093/oxfordjournals.jbchem.a134282" @default.
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