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- W9899785 abstract "The glycophorin A (GPA) assay utilizes immunofluorescent labeling and flow cytometry to measure the frequency of peripheral erythrocytes with mutant phenotypes, presumably due to mutations in erythroid precursor cells. Analysis of 5 [times] 10[sup 6] cells/assay is used to enumerate variant erythrocytes that occur at a frequency of 3-10 [times] 10[sup [minus]6] in unexposed donors. Extension of this assay to human reticulocytes requires detection of variants that occur at frequencies as low as 3 [times] 10[sup [minus]8]. The authors have used high speed data acquisition and cell classification electronics to perform 3-color analysis at rates up to 20,000 cells/s. High speed analysis of up to 10[sup 8] cells/assay has been used to enumerate GPA-variant reticulocytes in normal donors." @default.
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- W9899785 date "1993-01-01" @default.
- W9899785 modified "2023-09-27" @default.
- W9899785 title "High speed flow cytometric detection of rare glycophorin A mutations in human blood cells" @default.
- W9899785 hasPublicationYear "1993" @default.
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