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- W991105806 abstract "This study aimed to identify the secondary metabolites, in particular the class of diketopiperazines produced by the endophytic fungus Colletotrichum sp. isolated from stems of Senna spectabilis. The fungus was cultivated in differents liquidi culture media (PDB, YM, Malt Extract, Nutrient and Czapek) providing the respective crude extracts. These extracts were submitted to CCDC, HPLC-DAD and H NMR for metabolic production and evaluation of bioassays to assess the potential antifungal activity and acetylcholinesterase inhibition. The results indicate the PDB as the best medium for the endophyte growth on a large scale, whose extract was fractionated by column chromatography. The 12 fractions were obtained and subjected to HPLC-DAD and H NMR. The Fraction 1 was again subjected to fractionation by column chromatography and was identified 3 diketopiperazine (cyclo(Pro-Val), cyclo(Pro-Phe) and cyclo(Pro-Tyr)). The Fraction 01 also underwent dereplication using the simulation technique of H NMR spectrum with the aid of software MestreNova, and later to mass spectrometry, which enabled the identification of 5 diketopiperazines (cyclo(Pro-Ala), cyclo(Ile-Pro) or cyclo(LeuPro), cyclo(Val-Phe), cycle(Pro-Pro) and cyclo(Hyp-Ile)) and uracil." @default.
- W991105806 created "2016-06-24" @default.
- W991105806 creator A5071584785 @default.
- W991105806 date "2011-08-19" @default.
- W991105806 modified "2023-10-03" @default.
- W991105806 title "Avaliação química e biológica do fungo endofítico Colletotrichum sp. isolado de Senna spectabilis" @default.
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- W991105806 hasPublicationYear "2011" @default.
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