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- W991291295 abstract "This chapter describes a new silver impregnation method for nervous tissue in frozen sections of the human central nervous system in which ammoniacal silver-dichromate solution is used. The procedure of this method involves frozen sections that are 20-30 μm thick and are obtained from tissue blocks fixed in formalin. These are placed in ammoniacal silver-dichromate solution, 10% (w/v) aqueous solution of silver nitrate, and ammoniacal silver solution, which is then reduced in 20% (v/v) formalin, toned in 1% (w/v) aqueous solution of gold chloride, fixed in 5% (w/v) aqueous solution of sodium thiosulfate, dehydrated, and mounted. In this procedure, chromium (Cr) and silver (Ag) ions can be applied simultaneously to tissue sections and fine fibers display colors ranging from purple to dark purple on an almost colorless background. Gold treatment may be omitted. This procedure has given good results for the tissues that have been fixed in formalin for several years. The principal steps in common Golgi-type silver impregnation methods involve aldehyde fixation, immersion of tissue blocks in potassium dichromate, and subsequent immersion in silver nitrate to deposit precipitates in tissue blocks." @default.
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- W991291295 date "1990-01-01" @default.
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- W991291295 title "Silver Impregnation Method for Frozen Sections of Human Nervous Tissue Using Ammoniacal Silver–Dichromate Solution" @default.
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- W991291295 doi "https://doi.org/10.1016/b978-0-12-185255-9.50023-7" @default.
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