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- W991990986 abstract "Membrane proteins play central roles in many significant cellular processes such as signal transduction, cell adhesion and immune recognition reactions. As a result, they gained substantial pharmacological significance, which led to an increased effort to develop analytical technologies that enable characterisation of ligand binding to membrane proteins in membranes [1]. This thesis dealt with the development of membrane assay formats to address different issues concerning biomembrane sensing. Reflectometric interference spectroscopy (RIfS), a label-free optical technique was used as sensing method. RIfS is a versatile method for the height determination of thin transparent films, since the signal obtained by interference of multiple reflected partial white light beams contains information about the thickness and refractive index of a thin layer. It has already been proven in the past, that RIfS is a powerful measurement system for the detection and quantification of proteinprotein interactions [2]. Hence, we chose to explore the capabilities of the technique when applied to biomembrane sensing. For this purpose, two different set-ups were built to perform RIfS. A standard instrument and a set-up which combined the method with fluorescence microscopy. An assay format based on solid-supported membranes was developed, which allows the quantification of protein-protein and protein-lipid interactions on artificial, as well as reconstituted cellular membranes. Additionally, a transport assay was designed utilising pore-spanning membranes suspended over small cavities, which allows the simultaneous measurement of the outward and inward flow of molecules across a lipid membrane. Apart from the two membrane based assay formats, a third assay for the measurement of molecular recognition reactions of low-molecular-weight analytes was developed. The assay allows to directly quantify the binding of small analytes to their surface-immobilised partner." @default.
- W991990986 created "2016-06-24" @default.
- W991990986 creator A5016101273 @default.
- W991990986 date "2022-02-20" @default.
- W991990986 modified "2023-10-18" @default.
- W991990986 title "Development of a Biomembrane Sensor Based on Reflectometry" @default.
- W991990986 doi "https://doi.org/10.53846/goediss-4000" @default.
- W991990986 hasPublicationYear "2022" @default.
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