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- W992470113 abstract "Beta-mannanase,as a kind of hemicellulose enzyme,has great economic value and social significance.With the aim of providing experimental evidence for its industrial production by fed-batch fermentation,Bacillus licheniformics HDYM-04 was cultured in a 5 L fermentor and fermentation conditions,feeding strategy and the amount of konjac powder addition were optimized.The optimal initial konjac addition,initial pH and inoculum size were determined as 60 g/L,8.0 and 6.7%,respectively.The optimal fermentation conditions were temperature of 37 ℃,300 r/min of agitation speed,and 3 L/min of aeration rate.Initial konjac powder addition at a level of 30 g followed by 90 g during the late logarithmic growth phase was identified as the optimal feeding strategy.As a result,the β-mannanase activity in fermentation broth was up to 3913 U/mL,which was 89% higher than before the optimizations(2070 U/mL)." @default.
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- W992470113 date "2011-07-15" @default.
- W992470113 modified "2023-09-24" @default.
- W992470113 title "Optimization of Fed-batch Fermentation Conditions for Production of β-Mannanase by Bacillus licheniformics HDYM-04" @default.
- W992470113 hasPublicationYear "2011" @default.
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