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- W992938358 abstract "Publisher Summary This chapter describes how lac gene fusions have been used to study the MalF and MalK proteins, two cytoplasmic membrane components of the maltose transport system of Escherichia coli K-12. Membrane proteins which mediate the active transport of solutes are often difficult to detect, isolate, and characterize biochemically. Construction of lac gene gusions is a technique, which is use for fusing the 5´ end of any E. coli gene to the lacZ gene. After that many other techniques have been developed to fuse genes to lacZ. Some of these take advantage of the ease and flexibility with which one can manipulate the lac genes in vivo and others entail the use of recombinant DNA technology. Any of the techniques mentioned in this chapter can be used to generate lacZ gene fusions which retain β galactosidase activity. To illustrate how lacZ fusions have been used to identify and characterize transport proteins, the purification of a MalF-LacZ hybrid protein and its uses are described in the chapter." @default.
- W992938358 created "2016-06-24" @default.
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- W992938358 date "1986-01-01" @default.
- W992938358 modified "2023-09-27" @default.
- W992938358 title "[12] Use of 1ac gene fusions to study transport proteins" @default.
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- W992938358 doi "https://doi.org/10.1016/s0076-6879(86)25014-4" @default.
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