FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W99331475> ?p ?o ?g. }

• W99331475 endingPage "112" @default.
• W99331475 startingPage "108" @default.
• W99331475 abstract "Activation and clonal expansion of T cells require not only the recognition of processed antigen on the surface of the antigen-presenting cell (APC) by T-cell receptor (TCR), but also involve co-stimulatory signals that are provided by the simultaneous engagement of cell surface molecules expressed by both the APC and the T cell. Interaction between CD40 and its ligand (CD40L) is known to mediate host immune response and T-cell-mediated effector functions in mycobacterial infections in mice. In this work, we investigated the capacity of Mycobacterium bovis (M. bovis) BCG to induce the expression of CD40L on human T cells. Human cells were obtained from healthy adults by centrifugation using Ficoll/Hypaque. Cells (1 × 106) were incubated in RPMI medium with BCG. After incubation at 37°C in 5% CO2 atmosphere for 40 h, cells were collected and double-stained with anti-CD40L-PE and anti-CD4-FITC or anti-CD8-FITC mAb. The quantification of positively stained population was based on samples stained with isotype control antibodies analyzed on a FACScan. M. bovis BCG stimulation induced a significant amount of CD40L expression on CD4+ T cells, while CD40L was not significantly detected on human CD8+ T cells. The highest CD40L expression on BCG-activated T cells in synergism with interleukin-12 endogenous occurred after a 40-h cell culture with a dose of 10 μg/mL of BCG (84.86 ± 11.77; mean ± standard deviation [SD]). This CD40L expression on BCG-activated human T cells was significantly inhibited by anti-IL-12 mAb (5.08 ± 1.7; mean ± SD). In contrast, anti-IFN-γ and anti-IL-2 mAb did not have an important role in this expression. These results indicate that the capacity of BCG to induce CD40L expression on human cells represents a novel mechanism underlying the regulation of cellular responses against tuberculosis. Furthermore, the results showed a direct role of IL-12 to enhance the expression of CD40L on BCG-activated human cells." @default.
• W99331475 created "2016-06-24" @default.
• W99331475 creator A5000903764 @default.
• W99331475 creator A5029918782 @default.
• W99331475 date "2001-03-01" @default.
• W99331475 modified "2023-10-15" @default.
• W99331475 title "CD40 Ligand Expression in Mycobacterium bovis BCG Infection and Its Regulation by Cytokines" @default.
• W99331475 cites W1506273507 @default.
• W99331475 cites W1528551608 @default.
• W99331475 cites W1589722286 @default.
• W99331475 cites W1966820980 @default.
• W99331475 cites W1984643187 @default.
• W99331475 cites W2076174684 @default.
• W99331475 cites W2080674250 @default.
• W99331475 cites W2102156468 @default.
• W99331475 cites W2102632161 @default.
• W99331475 cites W2127729148 @default.
• W99331475 cites W2133128339 @default.
• W99331475 cites W2141342710 @default.
• W99331475 cites W2141676633 @default.
• W99331475 cites W2151925533 @default.
• W99331475 cites W2166067744 @default.
• W99331475 cites W2172960056 @default.
• W99331475 cites W4246021243 @default.
• W99331475 cites W4313342425 @default.
• W99331475 doi "https://doi.org/10.1016/s0188-4409(01)00258-2" @default.
• W99331475 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11343806" @default.
• W99331475 hasPublicationYear "2001" @default.
• W99331475 type Work @default.
• W99331475 sameAs 99331475 @default.
• W99331475 citedByCount "1" @default.
• W99331475 crossrefType "journal-article" @default.
• W99331475 hasAuthorship W99331475A5000903764 @default.
• W99331475 hasAuthorship W99331475A5029918782 @default.
• W99331475 hasConcept C147483822 @default.
• W99331475 hasConcept C153911025 @default.
• W99331475 hasConcept C154317977 @default.
• W99331475 hasConcept C167672396 @default.
• W99331475 hasConcept C202751555 @default.
• W99331475 hasConcept C203014093 @default.
• W99331475 hasConcept C2776090121 @default.
• W99331475 hasConcept C2908647359 @default.
• W99331475 hasConcept C39347974 @default.
• W99331475 hasConcept C55493867 @default.
• W99331475 hasConcept C67662055 @default.
• W99331475 hasConcept C71924100 @default.
• W99331475 hasConcept C79484868 @default.
• W99331475 hasConcept C86803240 @default.
• W99331475 hasConcept C8891405 @default.
• W99331475 hasConcept C99454951 @default.
• W99331475 hasConceptScore W99331475C147483822 @default.
• W99331475 hasConceptScore W99331475C153911025 @default.
• W99331475 hasConceptScore W99331475C154317977 @default.
• W99331475 hasConceptScore W99331475C167672396 @default.
• W99331475 hasConceptScore W99331475C202751555 @default.
• W99331475 hasConceptScore W99331475C203014093 @default.
• W99331475 hasConceptScore W99331475C2776090121 @default.
• W99331475 hasConceptScore W99331475C2908647359 @default.
• W99331475 hasConceptScore W99331475C39347974 @default.
• W99331475 hasConceptScore W99331475C55493867 @default.
• W99331475 hasConceptScore W99331475C67662055 @default.
• W99331475 hasConceptScore W99331475C71924100 @default.
• W99331475 hasConceptScore W99331475C79484868 @default.
• W99331475 hasConceptScore W99331475C86803240 @default.
• W99331475 hasConceptScore W99331475C8891405 @default.
• W99331475 hasConceptScore W99331475C99454951 @default.
• W99331475 hasIssue "2" @default.
• W99331475 hasLocation W993314751 @default.
• W99331475 hasLocation W993314752 @default.
• W99331475 hasOpenAccess W99331475 @default.
• W99331475 hasPrimaryLocation W993314751 @default.
• W99331475 hasRelatedWork W1982529683 @default.
• W99331475 hasRelatedWork W2000670206 @default.
• W99331475 hasRelatedWork W2051218772 @default.
• W99331475 hasRelatedWork W2106690765 @default.
• W99331475 hasRelatedWork W2146448606 @default.
• W99331475 hasRelatedWork W2153283302 @default.
• W99331475 hasRelatedWork W2538149674 @default.
• W99331475 hasRelatedWork W2744015319 @default.
• W99331475 hasRelatedWork W4313356390 @default.
• W99331475 hasRelatedWork W4313405642 @default.
• W99331475 hasVolume "32" @default.
• W99331475 isParatext "false" @default.
• W99331475 isRetracted "false" @default.
• W99331475 magId "99331475" @default.
• W99331475 workType "article" @default.