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- W993321 abstract "Publisher Summary This chapter examines the in vitro effects of natural killer cells on Cryptococcus neoformans (C. neoformans). In a series of experiments, NK cell reactivities of splenic cell populations were varied by nylon wool passage, varying donor mouse strains, using different aged mice, treating splenic cell donors with poly I:C or Corynebacterium parvum, and discontinuous Percoll gradient fractionation of the spleen cells. In each case, the NK reactivity of the effector cell pool was determined by using a 4 h 51Cr-release assay with YAC-1 targets with an effector:target ratio of 50:1, and the ability of the effector cells to inhibit the growth of cryptococci was measured by incubating C. neoformans isolate 184 with the effector cells at an E:T ratio of 500:1 for 18 h prior to enumerating the viable cryptococci by a plating technique. Although the numbers of C. neoformans cells increased in the assay vessels over the next 20 h, the % inhibition of cryptococci growth did not change significantly. It appears that the event that effects C. neoformans growth occurs relatively early in the incubation period and is detectable up to 24 h after mixing of effector and target cells." @default.
- W993321 created "2016-06-24" @default.
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- W993321 date "1982-01-01" @default.
- W993321 modified "2023-09-25" @default.
- W993321 title "IN VITRO EFFECTS OF NATURAL KILLER (NK) CELLS ON CRYPTOCOCCUS NEOFORMANS" @default.
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- W993321 doi "https://doi.org/10.1016/b978-0-12-341360-4.50163-3" @default.
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