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- W993608645 abstract "Although the problem of artifacts originating after blood collection has been recognized, there is no general awareness of this problem among all of the workers in the field. In view of this, the chapter describes various precautionary measures for collecting blood destined for lipoprotein isolation. Studies have shown that proteolytic enzymes of different types can affect the cleavage of proapoA-I, apoA-II, apoB, and apoE; for example, the enzyme responsible for the cleavage of proapoA-I to apoA-I is present in circulation and is inhibited by ethylenediaminetetraacetic acid (EDTA). The chapter proposes to develop a cocktail that is added to the bottle before blood collection to prevent the multiple enzymatic degradations that can occur during and after the withdrawal of blood. However, proper mixing of the cocktail with the collected blood is essential, because it will ensure that all constituents have come in contact and that preventive measures have begun. The chapter concludes that the lipoprotein distribution varies from individual to individual and is a characteristic of each normolipemic subject independent of time." @default.
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- W993608645 date "1986-01-01" @default.
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- W993608645 title "[5] Precautionary measures for collecting blood destined for lipoprotein isolation" @default.
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- W993608645 doi "https://doi.org/10.1016/0076-6879(86)28065-9" @default.
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