Matches in SemOpenAlex for { <https://semopenalex.org/work/W99367655> ?p ?o ?g. }
Showing items 1 to 57 of
57
with 100 items per page.
- W99367655 abstract "β-carotene monooxygenase (BCMO1) is a key enzyme in vitamin A biosynthesis in mammals. It symmetrically cleaves β-carotene to two molecules of all trans-retinal. The process of monooxygenation involves reductive oxygen activation at metal complexes and then oxygen transfer to substrate. Tyrosines play a key role in coupled proton-electron transfer in a large number of metalloenzymes. Therefore, we decided to investigate the role of tyrosines conserved among mammalian family members by oligonucleotide-directed mutagenesis. We found that while activities of Y235F, Y236F, Y418F, Y420F mutants of mouse BCMO1 were reduced, they all retained a substantial activity (more than 60% of wt activity). While change of Y235 and Y420 to leucines led to significant impairment of activity (less than 35% of wt), change of Y411, Y413 and Y418 to non-aromatic amino acids did not have a strong effect on BCMO1 activity. We also replaced the non-conserved Y326, predicted from our model to be located in the binding tunnel of mouse BCMO1. Changing to an aromatic (Y326W) had little influence on enzyme activity, while change to a non-aromatic residue (Y326L) substantially reduced activity. Therefore, we conclude that while the studied tyrosines are not essential for the catalytic mechanism, it seems that the presence of aromatic rings at Y235, Y326, Y420 is important for BCMO1 activity and they could be involved in electron transfer in BCMO1." @default.
- W99367655 created "2016-06-24" @default.
- W99367655 creator A5026999738 @default.
- W99367655 creator A5032376253 @default.
- W99367655 creator A5034742292 @default.
- W99367655 date "2007-04-01" @default.
- W99367655 modified "2023-09-30" @default.
- W99367655 title "Role of Tyrosines in Mouse β‐Carotene 15,15′‐Monooxygenase Activity" @default.
- W99367655 doi "https://doi.org/10.1096/fasebj.21.5.a274-c" @default.
- W99367655 hasPublicationYear "2007" @default.
- W99367655 type Work @default.
- W99367655 sameAs 99367655 @default.
- W99367655 citedByCount "0" @default.
- W99367655 crossrefType "journal-article" @default.
- W99367655 hasAuthorship W99367655A5026999738 @default.
- W99367655 hasAuthorship W99367655A5032376253 @default.
- W99367655 hasAuthorship W99367655A5034742292 @default.
- W99367655 hasBestOaLocation W993676551 @default.
- W99367655 hasConcept C103408237 @default.
- W99367655 hasConcept C104317684 @default.
- W99367655 hasConcept C13002179 @default.
- W99367655 hasConcept C143065580 @default.
- W99367655 hasConcept C16318435 @default.
- W99367655 hasConcept C181199279 @default.
- W99367655 hasConcept C185592680 @default.
- W99367655 hasConcept C526171541 @default.
- W99367655 hasConcept C55493867 @default.
- W99367655 hasConcept C71240020 @default.
- W99367655 hasConceptScore W99367655C103408237 @default.
- W99367655 hasConceptScore W99367655C104317684 @default.
- W99367655 hasConceptScore W99367655C13002179 @default.
- W99367655 hasConceptScore W99367655C143065580 @default.
- W99367655 hasConceptScore W99367655C16318435 @default.
- W99367655 hasConceptScore W99367655C181199279 @default.
- W99367655 hasConceptScore W99367655C185592680 @default.
- W99367655 hasConceptScore W99367655C526171541 @default.
- W99367655 hasConceptScore W99367655C55493867 @default.
- W99367655 hasConceptScore W99367655C71240020 @default.
- W99367655 hasIssue "5" @default.
- W99367655 hasLocation W993676551 @default.
- W99367655 hasOpenAccess W99367655 @default.
- W99367655 hasPrimaryLocation W993676551 @default.
- W99367655 hasRelatedWork W1572025105 @default.
- W99367655 hasRelatedWork W1964489770 @default.
- W99367655 hasRelatedWork W1975766203 @default.
- W99367655 hasRelatedWork W2013630470 @default.
- W99367655 hasRelatedWork W2053583806 @default.
- W99367655 hasRelatedWork W2077388906 @default.
- W99367655 hasRelatedWork W2124597856 @default.
- W99367655 hasRelatedWork W2279156773 @default.
- W99367655 hasRelatedWork W2351327234 @default.
- W99367655 hasRelatedWork W258580054 @default.
- W99367655 hasVolume "21" @default.
- W99367655 isParatext "false" @default.
- W99367655 isRetracted "false" @default.
- W99367655 magId "99367655" @default.
- W99367655 workType "article" @default.