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- W993832984 abstract "SummaryThe effects of explant size, pretreatment, and light intensity on shoot regeneration of apple (Malus × domestica Borkh.) were investigated using the rootstock genotype Mailing 7a. Young leaves were harvested from in vitro proliferating shoot cultures, sliced into sections or minced into small pieces, dipped in either sterile distilled water or liquid regeneration medium, and placed onto a medium consisting of half-strength Murashige and Skoog salts, Staba vitamins, 20 μ m thidiazuron, and 2.7 μ M tα -naphthaleneacetic acid. During the first four weeks of culture, explants were exposed to various light treatments: continuous darkness, two weeks of darkness followed by two weeks of low-light (15 μ mol m-2 s-1), continuous low-light, two weeks of low-light followed by two weeks of high-light (100 μ mol m-2 s-1), or continuous high-light. The regeneration frequency observed increased with decreasing light intensity and light duration. Mincing leaves and dipping explants in a liquid regeneration medium prior to culture also resulted in an increase in the number of shoots produced per leaf but did not significantly influence the regeneration frequency. The highest regeneration frequency (92%) was obtained when explants were sectioned, dipped in a liquid regeneration medium, and cultured in darkness for 4 weeks. However, the highest number of shoots (59.8 per leaf) was observed on minced explants cultured under the same conditions. A significantly higher regeneration frequency was obtained for five apple genotypes tested when leaves were minced rather than sectioned." @default.
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- W993832984 date "1994-01-01" @default.
- W993832984 modified "2023-09-26" @default.
- W993832984 title "Effects of explant size, pretreatment, and light intensity on shoot regeneration from in vitro-grown apple leaves" @default.
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- W993832984 doi "https://doi.org/10.1007/978-94-011-0467-8_71" @default.
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