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• W993854250 abstract "The chapter discusses the preparation and analysis of mitochondrial ribosomes. Mitochondria contain a distinct species of ribosome that functions in the synthesis of specific polypeptides of the inner mitochondrial membrane. The mitochondrial (mit) ribosomes of microorganisms and higher plants have sedimentation coefficients (s) of 70-80 S. Distinctive features of mit ribosomes include their sensitivity to inhibitors of bacterial protein synthesis, dissociation of monomers at relatively high Mg 2+ concentrations. Mit ribosomes are isolated from many organisms by procedures that include the following steps: (a) isolation of mitochondria, (b) lysis of mitochondria using either deoxycholate or a nonionic detergent, (c) preparation of a mit ribosomal pellet, and (d) separation of monomers or subunits on sucrose gradients. The chapter emphasizes two features: purification of the mitochondria by flotation gradient centrifugation and substitution of Ca 2+ for Mg 2+ during mitochondrial lysis to inhibit nuclease activity. A number of conventional electrophoretic systems are adapted for the analysis of mit ribosomal proteins." @default.
• W993854250 created "2016-06-24" @default.
• W993854250 creator A5005988075 @default.
• W993854250 date "1979-01-01" @default.
• W993854250 modified "2023-10-15" @default.
• W993854250 title "[34] Preparation and analysis of mitochondrial ribosomes" @default.
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• W993854250 doi "https://doi.org/10.1016/0076-6879(79)59103-4" @default.
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