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• W994978426 abstract "Publisher Summary L-type calcium channels mediate long-lasting calcium currents in response to strong depolarizations, and are the principal calcium channels in muscle tissues. This chapter focuses on purification and reconstitution of the L-type calcium channel from skeletal muscle. They are distinguished by their sensitivity to organic calcium channel modulators including the dihydropyridines (DHP), phenylalkylamines, and benzothiazepines. In skeletal muscle, L-type calcium channels mediate a slowly activated, long-lasting calcium current in the transverse tubules, which is blocked by DHP calcium channel antagonists. L-type calcium channel activity is observed at the single-channel level after reconstitution of purified channel preparations into planar bilayers. The results of ion flux, patch pipette, and macroscopic bilayer methods show that purified skeletal muscle calcium channels retain selective ion conductance activity that is modulated appropriately by calcium channel-modulating drugs and protein phosphorylation." @default.
• W994978426 created "2016-06-24" @default.
• W994978426 creator A5031055304 @default.
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• W994978426 date "1992-01-01" @default.
• W994978426 modified "2023-09-27" @default.
• W994978426 title "[35] Purification and reconstitution of skeletal muscle calcium channels" @default.
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• W994978426 doi "https://doi.org/10.1016/0076-6879(92)07037-o" @default.
• W994978426 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1382201" @default.
• W994978426 hasPublicationYear "1992" @default.
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