FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W99505366> ?p ?o ?g. }

• W99505366 endingPage "239" @default.
• W99505366 startingPage "225" @default.
• W99505366 abstract "Fluorescence-based sensing and imaging experiments are constrained by the background signal generated on a sample. A main contribution to the background, besides direct scattering of excitation and Raman scattering of the solvent, comes from sample autofluorescence and additives used for sample preparation. Such unwanted signals from endogenous chromophores and fixatives typically are broad and spectrally overlap with the probe signal; thus becoming a major limitation for sensitive detection and quantitative imaging. Since the fluorescence lifetimes of the majority of naturally occurring chromophores are relatively short, long-lived fluorophores allow for background discrimination by time-gated detection. Unfortunately, the brightness of long-lived, red-emitting fluorescent probes is inherently very low, consequently limiting many applications. Recently we reported a simple new approach with bursts of closely spaced laser excitation pulses for excitation (multi-pulse excitation) that allows for many-fold increase in the intensity of a long-lived probe over the background signal. This technology can be easily implemented for biomedical diagnostics and imaging to significantly enhance the signal of long-lived probes over the background. In this report, we are discussing an example of the Ruthenium-based dye tris(2,2′-bipyridyl)dichlororuthenium(II) hexahydrate (Ru) (Sigma–Aldrich) (~2% quantum yield and ~350 ns fluorescence lifetime) that when used with the multi-pulse approach and time-gated detection allows for high quality imaging that can easily be enhanced two orders of magnitude as compared to the normal approach (imaging with typical fluorescence microscopy)." @default.
• W99505366 created "2016-06-24" @default.
• W99505366 creator A5000604408 @default.
• W99505366 creator A5003671256 @default.
• W99505366 creator A5003998526 @default.
• W99505366 creator A5020510945 @default.
• W99505366 creator A5022216306 @default.
• W99505366 creator A5028671955 @default.
• W99505366 creator A5033121584 @default.
• W99505366 creator A5036867673 @default.
• W99505366 creator A5044967390 @default.
• W99505366 creator A5070834564 @default.
• W99505366 creator A5071855039 @default.
• W99505366 date "2014-01-01" @default.
• W99505366 modified "2023-09-27" @default.
• W99505366 title "Multiple-Pulse Pumping with Time-Gated Detection for Enhanced Fluorescence Imaging in Cells and Tissue" @default.
• W99505366 cites W1551656287 @default.
• W99505366 cites W1964440939 @default.
• W99505366 cites W1976102520 @default.
• W99505366 cites W1980154736 @default.
• W99505366 cites W1984580270 @default.
• W99505366 cites W1996067758 @default.
• W99505366 cites W2003179114 @default.
• W99505366 cites W2005977395 @default.
• W99505366 cites W2010922778 @default.
• W99505366 cites W2016857312 @default.
• W99505366 cites W2025397376 @default.
• W99505366 cites W2062169143 @default.
• W99505366 cites W2063497633 @default.
• W99505366 cites W2071179200 @default.
• W99505366 cites W2075048068 @default.
• W99505366 cites W2082454728 @default.
• W99505366 cites W2083266097 @default.
• W99505366 cites W2114560209 @default.
• W99505366 cites W2119893163 @default.
• W99505366 cites W2135434691 @default.
• W99505366 cites W2152213518 @default.
• W99505366 cites W2169813047 @default.
• W99505366 cites W2489486065 @default.
• W99505366 cites W2491458126 @default.
• W99505366 cites W2621096861 @default.
• W99505366 cites W287334105 @default.
• W99505366 cites W4256549968 @default.
• W99505366 doi "https://doi.org/10.1007/4243_2014_68" @default.
• W99505366 hasPublicationYear "2014" @default.
• W99505366 type Work @default.
• W99505366 sameAs 99505366 @default.
• W99505366 citedByCount "2" @default.
• W99505366 countsByYear W995053662022 @default.
• W99505366 countsByYear W995053662023 @default.
• W99505366 crossrefType "book-chapter" @default.
• W99505366 hasAuthorship W99505366A5000604408 @default.
• W99505366 hasAuthorship W99505366A5003671256 @default.
• W99505366 hasAuthorship W99505366A5003998526 @default.
• W99505366 hasAuthorship W99505366A5020510945 @default.
• W99505366 hasAuthorship W99505366A5022216306 @default.
• W99505366 hasAuthorship W99505366A5028671955 @default.
• W99505366 hasAuthorship W99505366A5033121584 @default.
• W99505366 hasAuthorship W99505366A5036867673 @default.
• W99505366 hasAuthorship W99505366A5044967390 @default.
• W99505366 hasAuthorship W99505366A5070834564 @default.
• W99505366 hasAuthorship W99505366A5071855039 @default.
• W99505366 hasConcept C120665830 @default.
• W99505366 hasConcept C121332964 @default.
• W99505366 hasConcept C125245961 @default.
• W99505366 hasConcept C147080431 @default.
• W99505366 hasConcept C169573571 @default.
• W99505366 hasConcept C179687394 @default.
• W99505366 hasConcept C185592680 @default.
• W99505366 hasConcept C192468462 @default.
• W99505366 hasConcept C192562407 @default.
• W99505366 hasConcept C199360897 @default.
• W99505366 hasConcept C2779843651 @default.
• W99505366 hasConcept C2780167933 @default.
• W99505366 hasConcept C40003534 @default.
• W99505366 hasConcept C41008148 @default.
• W99505366 hasConcept C57477423 @default.
• W99505366 hasConcept C62520636 @default.
• W99505366 hasConcept C75473681 @default.
• W99505366 hasConcept C83581075 @default.
• W99505366 hasConcept C91881484 @default.
• W99505366 hasConcept C94915269 @default.
• W99505366 hasConceptScore W99505366C120665830 @default.
• W99505366 hasConceptScore W99505366C121332964 @default.
• W99505366 hasConceptScore W99505366C125245961 @default.
• W99505366 hasConceptScore W99505366C147080431 @default.
• W99505366 hasConceptScore W99505366C169573571 @default.
• W99505366 hasConceptScore W99505366C179687394 @default.
• W99505366 hasConceptScore W99505366C185592680 @default.
• W99505366 hasConceptScore W99505366C192468462 @default.
• W99505366 hasConceptScore W99505366C192562407 @default.
• W99505366 hasConceptScore W99505366C199360897 @default.
• W99505366 hasConceptScore W99505366C2779843651 @default.
• W99505366 hasConceptScore W99505366C2780167933 @default.
• W99505366 hasConceptScore W99505366C40003534 @default.
• W99505366 hasConceptScore W99505366C41008148 @default.
• W99505366 hasConceptScore W99505366C57477423 @default.
• W99505366 hasConceptScore W99505366C62520636 @default.

• next