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- W996076872 abstract "Publisher Summary Recombination between the phage and bacterial circular chromosomes creates a single circular chromosome in which the viral DNA is inserted into the DNA of its host. The recombination that accomplishes the integration of the viral genome is limited to specialized sites on both the viral and host chromosomes. The viral site, attP, and the host site, attB, are functionally distinct. The two sites are however identical over a 15 base-pair region commonly called the attachment site core. Crossover between attP and attB occurs within this core, yielding a pair of hybrid sites, attL and attR, that flank the integrated viral or prophage DNA. A phage gene, int, is identified by mutations that depress λ lysogeny without affecting either the ability of the virus to infect cells or to repress the lytic viral functions. The int gene has been the subject of intensive genetic and physiological studies. This chapter focuses on the polypeptide, Int, encoded by int gene. Most purifications of Int have begun with cells in which the int gene is expressed from a mutant promoter. Promoting the integration-excision cycle of λ virus is regarded the primary function of Int protein. The ability of Int to promote site-specific recombination in vitro has been significantly valuable in studying the characteristics of attachment sites." @default.
- W996076872 created "2016-06-24" @default.
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- W996076872 date "1981-01-01" @default.
- W996076872 modified "2023-09-27" @default.
- W996076872 title "23 Site-Specific Recombination Protein of Phage Lambda" @default.
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- W996076872 doi "https://doi.org/10.1016/s1874-6047(08)60349-2" @default.
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