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- W996475286 abstract "This chapter discusses the determination of D-arabinokinase. In the course of assay, arabinose 5-phosphate and the adenine nucleotides were precipitated at the end of the reaction, and residual arabinose was measured with the orcinol reaction. One unit of D-arabinokinase is defined as the amount of enzyme, which will phosphorylate 1 micromole of D-arabinose per minute at 37°. Specific activity is expressed as units per milligram protein. During the purification the enzyme was quite unstable in solutions of low ionic strength. However, in the presence of 0.1 M (NH4)2SO4 even such dilute solutions as those eluted from the diethylaminoethyl (DEAE)-cellulose column maintained most of their activity for several days at 0°. Even the most purified preparations contained varying amounts of phosphoarabinoisomerase and kinases which phosphorylate D- and L-ribulose and D-ribose. Disruption of the cells in glycine buffer, pH 10.3 inactivated much of the phosphoarabinoisomerase. Large scale preparations of D-arabinose 5-phosphate can be carried out in the presence of phosphoarabinoisomerase if the phosphorylation is carried out at pH 10. The isomerase is completely inactive at this pH while the kinase has maximum activity." @default.
- W996475286 created "2016-06-24" @default.
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- W996475286 date "1966-01-01" @default.
- W996475286 modified "2023-09-25" @default.
- W996475286 title "[78] d-Arabinokinase" @default.
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- W996475286 doi "https://doi.org/10.1016/0076-6879(66)09090-6" @default.
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